摘要
对鱼类早期生长阶段的摄食研究有助于了解其饵料来源及其在食物网中的功能地位,而全面准确地获取其食物种类信息是关键,高通量测序技术的发展给动物食性研究带来了前所未有的机遇和挑战,.本研究以大亚湾人工码头海域金钱鱼稚鱼为对象,以18S rDNA为靶标分别使用传统种在稚鱼摄食研S究an中ge的r测序和适用性Illumina Solexa高通量测序对其食物组成进行分析,比较两方法.结果表明:金钱鱼稚鱼为杂食性,食物多样性高,纤毛虫和苔藓动物是最优势的食物类群盖.使用传统测序方法共获得67条有效食物序列,分属于8个类群,涵23个生物种类;使用高通量测序方法共获得17000多条有效食物序列,分属于9个类群,涵盖35个生物种类.两种方法检测到的食物类群基本相同,但高通量测序方法在反映食物多样性和覆盖范围上更具优势,且灵敏度更高,检测出传统测序方法未发现的甲藻和褐藻种类,说明高通量测序技术可以较全面而准确地覆盖稚鱼的食物谱.高通量测序获取的大量数据,可在一定程度上提供半定量信息,克服传统测序在定量研究方面的不足势更明显,食谱覆盖更广,检测灵敏度更高,显著提升了.高通量测序技术在稚鱼摄食研究上优数据与结果的可信度,可为海洋生物摄食生态学研究提供强有力的支撑.
Feeding analysis of fish at early growth stages is helpful to understand its food source and functional role. Comprehensively and accurately obtaining the information of food species is critical for this endeavor. The development of high-throughput sequencing（HTS） technology has brought unprecedented opportunities and challenges to dietary analysis of animals. Molecular techniques have been widely used for analyzing diet composition of different animals, among which HTS hasbeen success fully used on marine fish. But the accuracy and suitability of HTS have not been confirmed and compared with traditional Sanger sequencing on the dietary analysis of juvenile fish yet.In this study, a comprehensive comparison was performed between traditional Sanger sequencing and HTS on the diet analysis ofScatophagus argusjuveniles, an important commercial fish in South and Southeast Asia. The juvenile fish were collected near a wharf in Daya Bay, China, and analyzed both by traditional Sanger sequencing and HTS methods targeting 18 S rDNA gene. Results showed that the diet composition was complicated and dominated by ciliates and bryozoans, indicating an omnivorous feeding of juveniles. Sixty-seven effective sequences were obtained and 21 food species were identified by Sanger sequencing, while 35 food species from 17000 effective sequencings were identified by HTS. The food groups obtained by both techniques were similar. HTS had advantages in dietary coverage due to the large amount of data. HTS detected preys of dinoflagellates and brown algae which were not found in Sanger sequencing. In addition, HTS could provide semi-quantitative information to some extent based on a large amount of data, overcoming the deficiency of Sanger sequencing in quantitative research. Our results suggested that HTS is much more powerful than Sanger sequencing on diet analysis of juvenile fish, which can provide more details and comprehensive information about the feeding of juvenile fish.
作者
林先智
胡思敏
刘胜
黄晖
LIN Xian-zhi;HU Si-min;LIU Sheng;HUANG Hui(Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;University of Chinese Academy of Sciences,Beijing 100049,China;Tropical Marine Biological Research Station in Hainan,Chinese Academy of Sciences,Sanya 572000,Hainan,China）.)
出处
《应用生态学报》
CAS
CSCD
北大核心
2018年第9期3093-3101,共9页
Chinese Journal of Applied Ecology
基金
国家重点研发计划项目(2016YFC0502800)
广东省科技计划项目(2015A020216013,2017B030314052)
中国科学院战略性先导科技专项(XDA13020100)资助
