摘要
应用分子系统发育学的方法,以浙江沿海一海地瓜的18S rDNA和COI片段序列为分子标记,自行设计引物,进行了克隆,结合来自GenBank中10种海参的18S rDNA和COI同源序列,用邻接法和最大简约法构建系统进化树,并结合形态学特征对它们的分类地位进行了探讨。初步分析结果,基于此海地瓜的各形态学的研究,可以认为它属于海参纲芋参目;鉴于18S rDNA基因的分析,此海地瓜与拟刺参遗传距离为0.017亲缘关系非常近,可以认为它属于海参纲;从COI基因的角度分析,它虽然与怀玉参在系统树上聚成一枝,但支持率很低;仅为49%,遗传距离比较大,为0.223,不足以作为分析的依据。
The phylogenetic relation province were studied by analysis of of sea cucumber Acaudina moIpadioidea 1312 bp from 18S rDNA and 692 bp from gous sequences of ten species within Holothuroidea downloaded from GenBank cal characteristics. Molecular phylogenetic trees were Parsimony（MP）. The sea cucumber was found to be Morphotyping. The 18S rDNA gene analysis reveals and Pseudostichopus villosus is 0. 017, that Acaudina C to from the coast in Zhejiang COlmt DNA with homoloas well as the morphologionstructed by Neighbor-Joining（NJ）and Maximum Molpadiidae Holothuroidea, inferred from its Biothat the genetic distance between this sea cucumber molpadioidea belongs to Holothuroidea and is close to the Holothuroidea, Aspidochirota. Based on the COImt DNA gene analysis, there is close relationship between Acaudina molpadioidea and Pentamera lissoplaca, with long genetic distance of 0. 223, not being the basis for the phylogenetic position.
出处
《水产科学》
CAS
北大核心
2009年第12期733-736,共4页
Fisheries Science
基金
国家农业科技成果转化资金资助项目(2007GB2C220359)
宁波市科技成果转化资金资助项目(2007C100055)
宁波市科技局资助项目(2008C500265)
