摘要
为了研究屎肠球菌对哺乳期仔猪的益生作用,试验选用健康的杜×长×大新生仔猪9窝,将其随机分为对照组、屎肠球菌灭活菌组和屎肠球菌活菌组,每组3窝。从出生开始至6日龄,对照组、屎肠球菌灭活菌组和屎肠球菌活菌组仔猪分别经口灌服无菌脱脂乳、含屎肠球菌热灭活菌（浓度为1×108cfu/m L）的脱脂乳和含屎肠球菌活菌（浓度为1×108cfu/m L）的脱脂乳,10 m L/（头·d）。仔猪于7日龄开始饲喂教槽料,21日龄断奶。分别于7日龄和21日龄时从每窝中选出1头接近平均体重的仔猪屠宰,取肝脏和脾脏,采集空肠黏膜,计算并分析仔猪腹泻率、消化酶活性和脏器指数。结果表明：与对照组相比,灭活菌和活菌都不影响哺乳期仔猪的体重和日增重（P〉0.05）;屎肠球菌灭活菌组和屎肠球菌活菌组仔猪第3周的腹泻率都下降至0,整个哺乳期腹泻率分别下降77.44%（P=0.078）和92.03%（P〈0.05）,两个益生菌组间差异不显著（P〉0.05）;屎肠球菌灭活菌组和屎肠球菌活菌组的21日龄仔猪空肠麦芽糖酶活性分别增加9.67倍（P〈0.05）和11.29倍（P=0.01）,活菌还显著提高脂肪酶含量1.09倍（P〈0.05）,但两个益生菌组间差异不显著（P〉0.05）;灭活菌不影响仔猪肝脏指数和脾脏指数,活菌也不影响肝脏指数及7日龄仔猪脾脏指数,但显著降低21日龄仔猪脾脏指数（P〈0.05）。说明热灭活益生屎肠球菌对哺乳期仔猪的生长、腹泻率、小肠消化酶活性以及肝脏和脾脏指数都没有负面影响,而且在很大程度上与活菌的作用相似,具有替代活菌的潜力。
The aim of the present study was to investigate the probiotic effects of heat-killed probiotic Enterococcus faecium on sucking piglets. Nine litters of the newborn piglets （crossbred [ Large White ~ Landrace] and Duroc）were randomly divided into control group, heat-killed group and live bacteria group （three litters in each group）.From birth to 6 days of age, the piglets in the control group, heat-killed group and live bacteria group were orally given aseptic skimmed milk, skimmed milk containing probiotic heat-killed Enterococcus fizecium （ concentration 1 x l0s cfu/mL） and skimmed milk containing viable Enterococcus faecium （concentration 1 x 108 cfu/mL） , respectively （each pigletwas given lOml every （lay） .All piglets had free access to a creep feed from day 7 and were weaned on day 21 after birth. At the age of 7 days and 21 days, one piglet of nearly average hody weight was slaughtered from each litter of piglets.Then the Liver and spleen were taken out and the jejunal mu- cosa was collected. The diarrhea rate, digestive enzyme activity and organ index were calculated and analyzed.The results showed that compared with the control group, both heat-killed and live Enterococcus faecium （lid not significantly alt＇ect suckling piglet＇ s body weight and average dai- ly gain （P〉0.05）.The incidence of diarrhea in the third week of both theheat-killed and live Enterococcus faeciumgroup dropped off to zero. The dian＇hea rate of the whole suckling period decreased 77.44% （P = 0. 078 ） and 92.03% （P〈0. 05 ） , respectively. The difterence between the two groups was not significant （P〉0.05） .Compared with the control group, the activity of mahase in jejunum of 21 clay old piglets in heat- killed and live Enterococcus faecium group increased 9.67-fold （ P〈0.05 ） and 11.29-fold （ P = 0.01 ） , respectively. The live probioties also increased lipase content by 1.09-fold （P〈 0. 05 ）, but there was no significant difference between the two probiotics groups �
作者
李媛媛
郭乾鹏
梁世忠
向明
黄怡
LI Yuanyuan;GUO Qianpeng;LIANG Shizhong;XIANG Ming;HUANG Yi ＊(College of Animal Science and Technology,Guangxi University,Nanning 530004,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2018年第15期1-5,10,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31460607)
广西大学科研基金项目(XBZ120917
XJZ120285)
关键词
屎肠球菌
热灭活
仔猪
生长
腹泻率
消化酶活性
脏器指数
Enterococcus faecium
heat-killed
piglet
growth
diarrhea rate
digestive enzyme activity
viscera index
