摘要
为了探究SIF对肌肉生长的影响,我们用6周龄SD雄性大鼠作为实验对象,运用苏木精–伊红染色法、免疫组织化学法和实时荧光定量PCR法研究不同浓度大豆异黄酮(soybean isoflavone,SIF)作用下的雄性大鼠肌纤维形态学变化和α-肌动蛋白(α-actin)、肌球蛋白(myosin)及其m RNA表达量的变化。苏木精–伊红染色结果显示,中、高剂量组肌纤维直径极显著高于溶媒对照组(P<0.01)。免疫组织化学结果显示,中、高剂量组α-actin表达量极显著高于溶媒对照组(P<0.01);myosin表达量高剂量组极显著高于溶媒对照组(P<0.01),中剂量组显著高于溶媒对照组(P<0.05)。实时荧光定量PCR结果显示,目的基因α-actin和myosin m RNA的表达量变化与蛋白表达基本一致。以上结果表明,SIF能够通过促进α-actin和myosin蛋白及m RNA在肌肉中的表达,使雄性大鼠肌纤维增粗。可为大豆异黄酮改善肌肉收缩、促进骨骼肌纤维增粗提供理论指导和实验依据。
To investigate the effect of SIF on muscle growth, they used 6-week-old SD male rats as test subjects. We used hematoxylin-eosin staining, immunohistochemistry, and Real-time fluorescence quantitative PCR to study morphological changes of myofibers and changes in the expression of α-actin, myosin and m RNA in male rats after treatment with different concentrations of soybean isoflavone. The results of the study showed that the muscle fiber diameters of the middle and high dose groups were significantly higher than those of the vehicle control group（P〈0.01）. Immunohistochemical results showed that the expression of α-actin was significantly higher in the medium and high dose groups than in the vehicle control group（P〈0.01）; the high dose group of myosin was significantly higher than the vehicle control group（P〈0.01）, and the middle dose group was significantly higher than the vehicle control group（P〈0.05）. The results of real-time fluorescence quantitative PCR showed that the expression levels of target gene α-actin and myosin m RNA were basically consistent with the protein expression.The results showed that soybean isoflavones could thicken the muscle fibers by promoting the expression of actin, myosin and their m RNA in muscle, which could provide theoretical guidance and experimental basis for soybean isoflavone to improve muscle contraction and promote skeletal muscle fiber thickening.
作者
唐伊
谭金龙
罗启慧
黄超
刘文涛
陈正礼
Tang Yi;Tan Jinlong;Luo Qihui;Huang Chao;Liu Wentao;Chen Zhengli(Key Laboratory of Animal Disease and Human Health of Sichuan Province,College of Veterinary Medicine,Siehuan Agricultural University,Chengdu 611130,China;Institute of Preventive Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2018年第8期1319-1325,共7页
Chinese Journal of Cell Biology
基金
四川省大学生创新训练计划项目(批准号:201610626067)资助的课题~~
