摘要
目的采用培养法对血浆内毒素检测在艾滋病合并革兰阴性菌血症中的价值进行评价。方法选取艾滋病患者3 028例,采血行血需氧培养和内毒素检测,以培养出革兰阴性菌为标准,计算内毒素检测的诊断指标。结果在内毒素阳性组,25例培养出革兰阴性菌,387例未培养出革兰阴性菌,内毒素含量分别为(0.107±0.050)、(0.166±0.293)EU/mL(t=3.300,P=0.001);2 955例未培养出革兰阴性菌,73例培养出革兰阴性菌,内毒素含量分别为(0.044±0.116)、(0.056±0.048)EU/mL(t=1.712,P=0.090);以血培养出革兰阴性菌为标准,内毒素检测的敏感度、特异度分别为34.25%、86.90%。结论血浆内毒素检测在艾滋病合并革兰阴性菌血症中不是一项理想的诊断指标。
Objective To evaluate the value of plasma endotoxin test in AIDS patients with gram-negative bacteremia by culture method.Methods Blood samples from 3 028 AIDS patients were taken for aerobic culture and endotoxin test.Gram-negative bacteria were used as the standard to calculate the diagnostic index of endotoxin test.Results In the endotoxin positive group,25 cases cultured gram-negative bacteria and 387 cases did not cultured gram-negative bacteria.The endotoxin contents were(0.107±0.050)and(0.166±0.293)EU/mL(t=3.300,P=0.001).2 955 cases of Gram-negative bacteria were not cultured,and 73 cases of Gram-negative bacteria were cultured.The endotoxin contents were(0.044±0.116)and(0.056±0.048)EU/mL(t=1.712,P=0.090).The sensitivity and specificity of endotoxin test were 34.25%and 86.90%respectively based on gram-negative bacteria cultured in blood.Conclusion Plasma endotoxin test is not an ideal diagnostic indicator for AIDS complicated with gram-negative bacteremia.
作者
磨立达
罗晓璐
苏汉珍
梁超娟
MO Lida;LUO Xiaolu;SU Hanzhen;LIANG Chaojuan(Clinical Laboratory,the Fourth People′s Hospital of Nanning(the Nanning Infectious Disease Affiliated Hospital of Guangxi Medical University,Guangxi AIDS Clinical Treatment center(Nanning)),Nanning,Guangxi 530023,China)
出处
《国际检验医学杂志》
CAS
2018年第17期2140-2142,2146,共4页
International Journal of Laboratory Medicine
基金
广西壮族自治区卫生和计划生育委员会科研课题(Z2016067)
南宁市科学研究与技术开发项目(20163113)
关键词
艾滋病
培养法
内毒素
革兰阴性菌
菌血症
AIDS
culture method
endotoxin
gram negative bacteria
bacteremia