摘要
目的探讨特发性膜性肾病(IMN)患者尿外泌体miRNA-193a的表达变化。方法选取年龄、性别匹配的IMN肾病综合征患者(IMN肾病综合征组)、IMN缓解患者(IMN缓解组)各14例及正常对照组14名。利用Exoquick试剂盒提取三组尿样本外泌体,并提取尿外泌体中RNA,利用实时荧光定量逆转录聚合酶链反应(qRT-PCR)对miRNA-193a表达量进行测定。采用SPSS19.0软件进行统计学分析。结果三组间尿外泌体miRNA-193a的表达存在差异,以正常对照组的2-△△CT为1作为基准校正,IMN患者尿外泌体miRNA-193a的表达明显高于正常对照组(相对表达倍数为4.30±0.66),差异有统计学意义(t=4.579,P<0.05);其中IMN肾病综合征组尿外泌体miRNA-193a表达显著高于IMN缓解组(相对表达倍数6.07±0.72与2.54±0.43),差异有统计学意义(t=3.282,P<0.05)。结论IMN患者尿外泌体miRNA-193a表达显著高于正常组,并且随着疾病缓解表达下调。该结果提示尿外泌体miRNA-193a在IMN的发生发展过程中可能起到一定的作用。
Objective To explore the nfiRNA-193a expression in urine exosomes of idiopathic menlbranous nephropathy （IMN） patients, and to lay the foundation for studying the role of urinary- exosomes nfiRNA in development of IMN. Methods Age and gender-matched IMN patients and normal control were selected and divided into IMN nephrotic syndrome group, IMN renfission group, and normal control group with 14 cases each. Urine exosomes of IMN nehprotic patients, IMN complete or partial renfission patients, and heathy controls were isolated by ExoQuick Exosome precipitaion solution. Exosomal RNA was extracted by Trizol kits. The expression of nfiRNA-193a and nfiRNA-168a were measured by real-time qRT-PCR. Results The urine exosome miRNA-193a expression of IMN patients was much higher than that of healthy controls （ relative expression ratio 4.30 ± 0.66 vs 1.00, P 〈 0.05）. Meanwhile, the urine exosomal miRNA-193a expression of IMN nehprotic patients was significantly higher than that of IMN complete or partial remission patients （ relative expression ratio 6.07 ± 0.72 vs 2.54 ± 0. 43,P 〈 0.05）. Conclusions The urine exosome miRNA-193a expression of IMN patients was much higher than that of healthy controls, and downregulated as remission was achieved. The results suggested that miRNA-193a might play- a role in the pathogenesis of IMN.
作者
刘书馨
常明
刘杨
梁雪
康喆
Liu Shuxin;Chang Ming;Liu Yang;Liang Xue;Kang Zhe(Department of Nephrology,Dalian Municipal Central Hospital;Department of Ncphrology,Dalian Municipal Fifth Hospital,Dalian 116033,China)
出处
《中华肾病研究电子杂志》
2017年第2期69-72,共4页
Chinese Journal of Kidney Disease Investigation（Electronic Edition）
基金
辽宁省自然科学基金(2013020224)
大连市科技局计划项目(2014E14SF151)
