摘要
目的：分析完全不活动精子症患者精液参数与妊娠结局的关系。方法：选用2015年1月～2016年6月来我院就诊的40例完全不活动精子症患者作为实验组和100例精液常规参数正常者作为对照组。按照WHO第五版伏类精液检查与处理实验室手毋崂标准，采用计算机辅助精液分析系统进行精液常规检测，分析参数包括精液量、精子浓度、PH值，采用伊红一苯胺黑染色法计数精子存活率及人工镜检观察精子形态，同时统计其妊娠结局。结果：实验组与对照组相比，精子浓度有显著性差异（P〈0．01）。PH值有统计学差异（P〈0．05），精液量无统计学差异（P〉0．05）。其中40例完全不活动精子症患者伊红染色结果在1％-92％之间，未见完全死亡精子病例。精子形态学中发生断尾者占32．5％。实验组单精子卵胞浆内显微注射（intracyto—plasmic sperm injection，ICSI）妊娠率为77．‰。结论：不活动精子症患者常多伴有精子浓度减低、PH值下降及断尾的发生。但通过精子伊红染色观察，其真实存活率较高。大多可以通过ICSI技术进助孕，且妊娠结局与精予存活率没有明显相关性。
Objective：To analyze the relationship between semen parameters and pregnancy outcome in patients with fully inactive spermatozoa. Methods： Forty patients with completely inactive spermatozoa who were treated in our hospital from January 2015 to June 2016 were selected as the test group, and 100 patients with normal semen parameters were selected as the control group. According to Laboratory Manual for Human Semen Examination and Handling in the fifth edition of WHO, routine semen testing is performed by computer-assisted semen analysis system. The parameters included semen volume, sperm concentration, pH value, sperm livability counted by Eosin-Nigrosine staining method and sperm morphology observed by artificial microscopy. At the same time, we record their pregnancy outcomes. Results： Compared with the Control group, sperm concentration was significantly different （P〈0.01）, the pH value has statistical difference （P〈0.05）, the semen volume has no statistical difference （P〉0.05）in the test group. The Eosin-Nigrosine staining results of 40 patients with completely inactive spermatozoa is range from 1% to 92%, no cases of sperm complete death is found, and Sperm occurred off the tail in morphology accounted for 32.5%. The clinical pregnancy rate was 77.5% in the test group. Conclusion： Inactive spermatozoa often accompanied by lower sperm concentration, decreased pH and occurred off the taill But the real survival rate is higher by eosin staining. Most patients can be assisted by ICSI technology, and pregnancy outcomes and sperm survival rate was not significantly correlated.
作者
谢楠
李婷
陈力
杨斯桀
刘雯
高选
XIE Nan;LI Ting;CHEN Li(Reproductive medicine research center of Shandong university,Shandong Jinan 250001)
出处
《医学检验与临床》
2017年第12期25-27,共3页
Medical Laboratory Science and Clinics
关键词
完全不活动精子
精子浓度
精子活率
断尾
妊娠结局
Completely inactive sperm
Sperm concentration
Sperm survival rate
Tail off
Pregnancy outcome
