摘要
目的建立气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)测定人体血液中的毒鼠强的方法。方法血液样品用乙酸乙酯提取后,用GC-MS进行测定。通过单离子检测扫描模式(single ion monitoring,SIM)确定毒鼠强的特征离子进行定性,并以毒鼠强的3个特征离子(m/z121、m/z212、m/z240)作为母离子,使用不同的碰撞能进行碰撞,得到相应的子离子。选择分别来自3个母离子的离子对,以响应值较高的离子对作为定量离子对,其余2个离子对作为定性离子对,采样选择反应监测(selective reaction monitoring,SRM)方式进行测定。结果毒鼠强浓度范围在0.01~0.2μg/m L范围内呈正相关,相关系数r为0.9998,回收率为92.0%~104.5%,相对标准偏差(relative standard deviation,RSD)为3.62%~5.32%,血液样品的检出限为0.0005μg/m L。结论该方法灵敏度高,选择性好,检出限低,适合食物中毒人体血液中毒鼠强的痕量检测。
Objective To establish a method for determination of tetramine in human blood by gas chromatography-mass spectrometry（GC-MS）. Methods The blood sample was extracted with ethyl acetate, and determined by GC-MS. The characteristic ions of tetramine were determined by single ion monitoring（SIM）, and three characteristic ions（m/z121, m/z212 and m/z240） of tetramine were used as the parent ions, and different collision energy was used to collide. Ion pairs from 3 parent ions were selected, and ion pairs with higher response values were used as quantitative ion pairs. The other 2 ion pairs were used as qualitative ion pairs, and the selective reaction monitoring（SRM） method was used for measurement. Results There was a positive correlation between the concentration of tetramine in the range of 0.01-0.2 μg/m L, with the correlation coefficient（r） of 0.9998. The recoveries were 92.0%-104.5%, relative standard deviation（RSD） were 3.62%-5.32%, and the limit of detection of blood samples was 0.0005 μg/m L. Conclusion This method has high sensitivity, good selectivity and low detection limit, which is suitable for trace detection of tetramine in human blood.
作者
赵丽
段毅宏
师真
李彦生
刘建辉
ZHAO Li;DUAN Yi-Hong;SHI Zhen;LI Yan-Sheng;LIU Jian-Hui(Kunming Center for Disease Control and Prevention,Kunming 650228,China;Yunnan Provincial Center for Disease Control and Prevention,Kunming 650022,China)
出处
《食品安全质量检测学报》
CAS
2018年第15期4085-4088,共4页
Journal of Food Safety and Quality
关键词
毒鼠强
人体血液
食物中毒
气相色谱-质谱法
tetramine
human blood
food poisoning
gas chromatography-mass spectrometry
