摘要
课题组基于1H-NMR研究人参煎对高糖高脂饲料加小剂量链脲佐菌素（STZ）联合诱发的胰岛素抵抗（IR）模型大鼠胰腺核磁代谢组的影响。该实验将48只SD雄性大鼠随机分为正常组、模型组、人参煎组（3.76 g·kg^-1）和格列美脲组（0.04mg·kg^-1）,采用喂养高脂高糖饲料＋腹腔注射小剂量STZ引发2型糖尿病胰岛素抵抗模型,造模后分别给予高、中、低剂量的人参煎汤剂和药物格列美脲,8周末取各组大鼠胰腺组织,一定方法处理后采集^1H-NMR谱,并用主成分分析方法对其进行分析。与正常组比较,模型组的亮氨酸/异亮氨酸、缬氨酸、乳酸、肌酸含量显著升高（P〈0.01）,肌酸、胆酸、牛磺酸含量有变化,但没有显著性差异;肌醇含量明显降低（P〈0.001）。与模型组比较,人参煎组大鼠的亮氨酸/异亮氨酸、缬氨酸、丙氨酸、肌酸、胆碱、牛磺酸含量显著降低（P〈0.01）,乳酸及肌醇含量升高,显著性较小（P〈0.01）。结果表明人参煎可以调控糖尿病大鼠胰腺的氨基酸水平,促使新陈代谢回归到正常范围。基于^1H-NMR代谢组学以及对代谢标志物的分析能够模拟胰岛素抵抗大鼠的代谢变化,为人参煎治疗胰岛素抵抗的机制研究提供新的思路和方法。
Pancreas metabonomic profiles of the type 2 diabetic rats＇ induced by streptozotocin（STZ） and high-sugar,high fat diet on the treatment of Renshenjian decoction（RSJD） after 8 weeks were investigated. In this study,48 Rats were randomly divided into four groups： normal control（NC）,Pathological model（PM）,Renshenjian decoction（RSJD 3. 76 g·kg^-1） and glimepiride control（GC 0. 04 mg·kg^-1）. They are induced insulin resistance model of type 2 diabetes mellitus by streptozotocin（STZ） after 4 weeks＇ high-sugar,high fat diet except for NC. After sucessful modeling,they are given intragastric administration respectively with same amount of saline,RSJD and glimepiride in 4 weeks. At the end of the 8 th week,the pancreatic tissue of rats in each group was collected,and the ^1H-NMR spectrum was collected after being treated by certain method,and analyzed by principal component analysis（PCA）. Compared with NC＇s rats,we found PM＇s a significant elevation in the level of leucine/isoleucine,valine,lactic acid,creatine but reduction in the level of inose and less obvious changes in the level of creatine,cholic acid,taurine in pancreatic extract. After having been recieved RSJD,reduction level in leucine/isoleucine,valine,alanine,creatine,choline,taurine are also found in pancreatic extract of RSJD＇s rats,together with the increase of creatinine and tryptophan levels. The results showed that RSJD could regulate the level of amino acids in pancreas of IR rats,promoting a recovery in the process of metabolism. It＇s helpful to simulate the metabolic changes of IR rats via^1H-NMR for a further understanding to study the mechanism how RSJD treat IR rats.
作者
郑惠婷
李克宁
陈驰
林卫东
王淑美
ZHENG Hui-ting;LI Ke-ning;CHEN Chi;LIN Wei-dong;WANG Shu-mei(Guangdong Pharmaceutical University,Guangzhou 510006,China;Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica,State Administration of Traditional Chinese Medicine,Guangzhou 510006,China;Chinese Medicine Quality Engineering Technology Research Center of Guangdong Province/Higher-Education,Guangzhou 510006,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第14期3012-3017,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81773884)
