摘要
灰霉病是滇重楼的主要病害之一,常引起滇重楼花器腐烂而导致种子绝收,该研究通过田间病害调查,掌握了该病发生的基本情况,通过带病组织和菌核培养分离获得病原菌,并依据柯赫氏法则验证其致病性。结果表明,病原菌分生孢子梗葡萄状分枝,分生孢子椭圆形,长9.70~13.70μm,平均（11.32±0.82）μm,宽（7.05~9.12）μm,平均（8.24±0.48）μm,精孢子球形,直径（3.34±0.31）μm,病原菌在发病植株及PDA培养基上均易产生黑色菌核,基于形态特征鉴定病原菌为一种葡萄孢属Botrytis真菌。基于病原菌RPB2,HSP60,G3PDH基因及其联合基因构建的系统发育树,支持该病原菌为葡萄孢属真菌一个新种,该菌最适培养温度为20℃,最适pH为8,持续光照会抑制病原菌生长。
Gray mold disease is one of the most important diseases of planted Paris polyphylla var. yunnanensis,the disease appeared primarily as blossom blights and fruit rots,but also as stem rots,leaf rots. In this study,the pathogenetic fungi was isolated from plant tissue or sclerotia that covering the fruit of diseased P. polyphylla var. yunnanensis,the pathogen was certified according to Koch＇s Postulation. The pathogen produced abundant black,irregular sclerotia on surface of diseased plants and potato dextrose agar.The conidiophores and clusters of oval conidia resembled a grape-like cluster,the size of conidia was 9. 70-13. 70 μm [average of（11. 32 ± 0. 82） μm]× 7. 05-9. 12 μm [average of（8. 24 ± 0. 48） μm],the microconidia produced on potato dextrose agar were spherical,and the size was（3. 34 ± 0. 31） μm,the pathogen was identified as Botrytis sp based on morphological characteristics. The DNA sequence analysis of the G3 PDH,HSP60,RPB2 genes placed the pathogen in a single clade that outside defined species of Botrytis,so the pathogen could be identified as a new species of Botrytis. The pathogen requires 20 ℃,pH 8,darkness or low light condition for the best growth.
作者
马维思
陈君
严世武
王馨
董志渊
李林玉
杨丽英
杨斌
MA Wei-si;CHEN Jun;YAN Shi-wu;WANG Xin;DONG Zhi-yuan;LI Lin-yu;YANG Li-ying;YANG Bin(Institute of Medicinal Plants,Furman Academy of Agricultural Sciences,Kunming 650205,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100193,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第14期2918-2927,共10页
China Journal of Chinese Materia Medica
基金
云南省应用基础研究计划青年项目(2017FD201)
云南省科技惠民计划项目(2016RA057)
中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-017)
云南省科技创新人才计划项目(2015HB102)
关键词
滇重楼
灰霉病
病原鉴定
葡萄孢
Paris polyphylla var. yunnanensis
grey mould disease
pathogen identification
Botrytis sp.
