摘要
Aim The several species of the genus Paris called ＂Chonglou＂ are famous traditional Chinese herbal medicines. We established the quantitative analysis method of the steroidal saponins in some species of the genus Paris and discussed their relations. Methods We detected the contents of 11 steroidal saponins in Paris samples with a Kromasel C18 （ 150 mm× 4.6 mm ID, 5μm） column which was subjected to gradient elution with acetonitrile-water （30：70- 60：40, V/V） at a flow rate of 1 mL· min^-1 by HPLC-ELSD and established chemical cluster tree using SPSS 11 software. Results All the samples could be separated and calibration curves of 11 saponins were prepared. We successfully detected the contents of 11 steroidal saponins in 14 Paris spp. in 30 min. The recovery for the assay of saponins was between 95 % and 97 %. The RSD of precision of 11 saponins and stability of samples were below 3 %. Chemical phylogenetic tree based on saponin contents indicated that 17 samples of Paris spp. clustered separately. Conclusion The established method is accurate and convenient, and suitable for the quantitative analysis of these 11 steroidal saponins in Paris spp.. The chemical phylogenetic tree is in accordance with Takhtajian classical taxonomy.
目的重楼是中国的传统名贵中药,建立重楼属植物甾体皂苷的含量分析方法,并在此基础上探讨它们的亲缘关系。方法应用蒸发光散射检测器,结合梯度洗脱程序,以乙腈水(3070～6040,VV)体系为流动相,流速为1mL·min-1,在C18(150mm×4.6mmID,5μm)柱上,测定重楼药材中11种甾体皂苷的含量,并用SPSS11软件构建重楼属植物化学聚类图。结果在30min以内,成功地对14种重楼属植物(共17个样本)进行甾体皂苷含量测定,根据它们皂苷含量上的特征对17个样本分别进行化学聚类。精密度、稳定性的RSD均小于3%,加样回收率在95%～97%之间。结论此方法对这11种甾体皂苷的测定具有良好的精密度,可用于重楼药材中主要活性成分甾体皂苷的定量分析。在此基础上构建的化学聚类树能得到塔赫他间分类系统的支持。
