摘要
目的对不同种质黄芩药材的高效液相色谱(HPLC)指纹图谱进行比较研究。方法采用HPLC法对10种不同种质黄芩样品进行测定。利用中国药典委员会颁布的《中药色谱指纹图谱相似度评价系统》(2004 A版)对其进行相似度评价;利用SPSS软件对其进行聚类分析。结果 10种不同种质黄芩药材的指纹图谱共检出11个共有峰,相似度较高,聚类分析可分为三大类。结论不同种质黄芩主要有效成分之间的比例存在较大差异,可利用HPLC指纹图谱技术评价不同种质黄芩药材。
Objective To establish a HPLC method for comparative study on HPLC fingerprints of different germplasm Scutellaria baicalensis Georgi. Methods 10 different germplasm samples of Scutellaria baicalensis Georgi were determined by HPLC. The similarity evaluation is carried on different germplasm of Scutellariae Radix by using similarity evaluation system for chromatographic fingerprint of TCM（ Version 2004 A） and cluster analysis was carried out by using SPSS software. Results A total of 11 common peaks were detected in the fingerprints of 10 different germplasm Scutellaria baicalensis Georgi,and the similarity was higher. The cluster analysis can be divided into three categories. Conclusion The proportion of main active ingredients in Scutellaria baicalensis Georgi is different,and HPLC fingerprint technology can be used to evaluate the different germplasm of Scutellaria baicalensis Georgi.
作者
管仁伟
王幼鹏
魏丕振
吕玲霞
辛立红
林慧彬
林建强
GUAN Ren-wei;WANG You-peng;WEI Pi-zhen;LV Ling-xia;XIN Li-hong;LIN Hui-bin;LIN Jian-qiang(Shandong University of Traditional Chinese Medicine,Jinan Shandong 250355,China;Linyi Health School of Shandong Province,Linyi Shandong 276000,China;Shandong Fangjian Pharmaceutical Co.,Ltd.,Jining Shandong 272071,China;Shandong Academy of Chinese Medicine,Jinan Shandong 250014,China;State Key Lab of Microbial Technology,Shandong University,Jinan Shandong 250100,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2018年第5期1102-1104,共3页
Lishizhen Medicine and Materia Medica Research
基金
山东省自然科学基金(ZR2017MH023
ZR2018PH040)
国家重点研发计划(2017YFC1701500
2017YFC1701502
2017YFC1701504)
山东省科技攻关计划(2014GSF119018
2015GSF119011)
中医药公共卫生服务补助专项“全国中药资源普查项目”(财社[2017]66号)
山东省第三批五级中医药师承教育项目
泰山学者工程(NO.ts201511107)
中央本级重大增减支项目-名贵中药资源可持续利用能力建设(2060302)
山东
关键词
黄芩
种质
高效液相色谱指纹图谱
Scutellaria baicalensis Georgi
Germplasm
HPLC fingerprint
