摘要
目的观察褪黑素静注对缺血性脑卒中大鼠脑损伤的修复作用。方法 36只SD大鼠随机法分为药物组、模型组、对照组各12只,药物组、模型组大鼠均制备缺血性脑卒中模型,对照组大鼠只暴露动脉不闭塞血管;药物组大鼠制模后5 min、24 h、48 h尾静脉注射6 mg/mL褪黑素,模型组制模后同各时点注射等量含有4%酒精的生理盐水。制模28 d后各组大鼠灌注取脑,并行冰冻切片,比较各组大鼠脑梗死体积、脑组织凋亡细胞数量、损伤区脑组织新生神经元前体细胞数量、损伤区脑组织新生成熟神经元数量。结果药物组和模型组大鼠脑组织损伤区域呈灰白色,对照组脑组织呈红色;药物组、模型组、对照组大鼠脑梗死体积百分比分别占20.09%±2.17%、32.11%±4.65%、0,药物组与模型组比较,P<0.05。药物组、模型组、对照组凋亡指数分别为7.51%±1.21%、14.12%±1.89%、1.00%±0.12%,药物组分别与模型组、对照组比较,P均<0.05。药物组大鼠脑组织损伤区域可见到较多的DCX/Brd U双标阳性的神经元前体细胞,模型组脑组织损伤区域可见到少量DCX/Brd U双标阳性的神经元前体细胞,对照组大鼠脑组织几乎未见到DCX/Brd U双标阳性的神经元前体细胞;药物组、模型组、对照组DCX/Brd U双标阳性神经元前体细胞分别占14.01%±2.89%、5.02%±1.61%、0,模型组分别与药物组、对照组比较,P均<0.05。药物组大鼠脑组织损伤区域可见到较多的Neu N/Brd U双标阳性的神经元,模型组大鼠脑组织损伤区域可见到少量Neu N/Brd U双标阳性的神经元,对照组大鼠脑组织几乎未见Neu N/Brd U双标阳性的神经元;药物组、模型组、对照组Neu N/Brd U双标阳性新生成熟神经元分别占10.58%±1.02%、2.25%±0.18%、0,药物组与模型组比较,P<0.05。结论褪黑素能够保护缺血脑损伤区细胞免于凋亡,并促进损伤区新生神经元的分化,从而促进缺血性脑卒中大鼠脑损伤的修�
Objective To observe the repair effect of melatonin on brain damage in ischemic stroke rats. Methods Thirty-six Sprague-Dawley（ SD） rats were randomly divided into the drug group,model group,and control group,with 12 in each. The ischemic stroke model was prepared in the drug group and the model group. We only exposed the arterial nonocclusive blood vessels of rats in the control group. The rats in the drug group were injected with 6 mg/mL melatonin through the tail vein at 5 min,24 h,and 48 h after molding. The rats in the model group were injected with the same amount of normal saline containing 4% alcohol at each time point. After 28 days of molding,the rats in each group were sacrificed and brains were taken. The brain was frozen and sliced. The infarct volume,the number of apoptotic cells in the brain,the number of neonatal precursor cells in the brain tissues,and the number of newly mature neurons in the brain tissues were compared. Results The brain tissue damage area of the drug group and the model group was grayish white,and the brain tissue of the control group was red. The percentage of cerebral infarction volume in the drug group,model group,and control group accounted for 20. 09% ± 2. 17%,32. 11% ± 4. 65%,and 0,respectively,and significant difference was found between the drug group and the model group（ P〈0. 05）. The apoptotic indexes of the drug group,the model group,and the control group were 7. 51% ± 1. 21%,14. 12% ± 1. 89%,and 1. 00% ± 0. 12%,respectively; significant difference was found between the drug group and the model group,and between the drug group and the control group,all P〈0. 05. In the drug group,more DCX/Brd U double positive neuron precursor cells were observed in the brain injury area,and a small number of DCX/Brd U double positive neuron precursor cells were observed in the model group. There were few DCX/Brd U double positive neuronal precursor cells in rat brain tissues of the control group. The DCX/Brd U double positive neuron precursor cells in the drug group
作者
衣昕
刘慧慧
肖国栋
YI Xin1, LIU Huihui, XIAO Guodong(1 Medical college of Nantong University, Nantong 226001, Chin)
出处
《山东医药》
CAS
2018年第27期22-25,共4页
Shandong Medical Journal
基金
中匈科学合作基金-青年学者计划项目(HCSCF-2017-3)
