摘要
目的探讨褪黑素(MT)对脂多糖(LPS)致脑损伤幼鼠脑组织胶质纤维酸性蛋白(GFAP)表达的影响及意义。方法160只1月龄SD大鼠随机分为LPS组、9g/L盐水组(NS组)、MT保护A组(MT加LPS,提前15min予MT)及MT保护B组(MT加LPS,即时给予MT)4组,每组40只。各组均按观察时间分为24、48h2个时间点,每组各时间点均为20只,其中均有10只不注射伊文思蓝(EB),观察至相应时间点取其脑组织。以甲酰胺法检测其脑组织EB水平,免疫组织化学染色法检测其GFAP表达,采用上海山富数字图像分析系统进行半定量分析。结果LPS组脑组织EB水平、GFAP阳性区面积与NS组各相应时间点比较差异均有统计学意义(Pa<0.01);MT保护组EB水平、GFAP阳性区面积均显著高于NS组(Pa<0.01);MT保护组EB水平、GFAP阳性区面积均显著低于LPS组(Pa<0.01);MT保护组组间比较无显著性差异(P>0.05)。结论LPS可诱导内毒素致大鼠感染性脑损伤GFAP的表达;MT可能在治疗感染性脑损伤中发挥作用。
Objective To explore the inhibitory effect of melatonin（MT） on expression of glial fibrillary acidic protein in infant rats with brain injury induced by lipopolysaccharide（LPS）.Methods One hundred and sixty Spragne-Dawley rats（1 month） were randomly divided into 4 groups：9 g/L saline group（NS group）,LPS group,MT protective A group（MT plus LPS,MT was given 15 minutes earlier）,and MT protective B group（MT plus LPS,MT was given immediately）.Forty rats in each group,then every group was subdivided into 2 subgroups according to observe time point at 24 h and 48 h with 20 rats,respectively.Half of them in all subgroups were not injected Evans blue（EB）.Rats were sacrificed at 2 time points to take brain tissue samples,respectively.EB content of brain tissue was meteraged by the formamide methods.Immunohistochemistry technology and computer assisted image analysis system methods were applied to observe the expression of the positive apoptosis cell of brain tissue.Results Both EB content and the expression of GFAP showed significant difference between LPS group and NS group（Pa〈0.01）;The expression of positive GFAP area and EB content in MT protective groups were much higher than those in NS group （Pa〈0.01）;The expression of positive GFAP area and EB content in MT protective groups were significantly lower than those in LPS group （Pa〈0.01）;The expression of positive GFAP area and EB content in MT protective subgroups were not significantly different （P〉0.05）.Conclusions LPS can induce the expression of GFAP in infectious brain injury of rats induced by LPS,and MT can inhibit the expression of GFAP;MT may play a role in dealing with infectious brain injury.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2009年第13期1014-1015,1018,共3页
Journal of Applied Clinical Pediatrics
关键词
褪黑素
脂多糖
胶质纤维酸性蛋白
幼鼠
melatonin
lipopolysaccharide
glial fibrillary acidic protein
infant rat
