摘要
目的:建立HPLC法测定大鼠血浆药物浓度,探讨不同剂量复方苦参注射液在大鼠体内对多西他赛多次给药后药物代谢动力学的影响。方法:将24只SD大鼠随机分为高、中、低剂量组和对照组,每组6只,大鼠尾静脉注射分别给予2倍临床等效量(3.6 m L/kg)、临床等效量(1.8 m L/kg)、1/2倍临床等效量(0.9 m L/kg)的复方苦参注射液和0.9%氯化钠注射液,连续给药7 d,第7天给予复方苦参注射液或0.9%氯化钠注射液5 min后,注射多西他赛15 mg/kg,按时间点采集血样处理并检测分析,计算药物代谢动力学参数。结果:多次给药后高剂量组、中剂量组AUC、Cmax均低于对照组(P<0.05),低剂量组与对照组差异无统计学意义(P>0.05)。结论:复方苦参注射液联合使用多西他赛后血药浓度有所降低,对多西他赛的抑制作用具有剂量依赖性,可为临床用药提供一定参考。
Objective： To develop a high performance liquid chromatography（ HPLC） method for the determination of docetaxol concentration,and investigate the effects of different dosages of compound Kushen injection on the pharmacokinetics of multiple-dose docetaxol in rats. Methods： Twenty-four SD rats were randomly divided into the high-dose,medium-dose,low-dose and control groups（ 6 rats each group）. The high-dose,medium-dose,low-dose and control groups were injected with 2 times clinical equivalent compound Kushen injection（ 3. 6 m L/kg）,clinical equivalent compound Kushen injection（ 1. 8 m L/kg）,1/2 clinical equivalent compound Kushen injection（ 0. 9 m L/kg） and 0. 9% normal saline solution by caudal vein for 7 days,respectively,and all rats were injected with 15 mg/kg of docetaxol after 5 min of administration on the seventh day. The blood samples in four groups at different time-points were analyzed,and the pharmacokinetic parameters were calculated. Results： After multiple-dose,the AUC and Cmaxin high-dose and medium-dose groups were lower than those in control group（ P〈0. 05）,and the difference of which between low-dose group and control group was not statistically significant（ P〉0. 05）. Conclusions： The compound Kushen injection can reduce the blood concentration of docetaxol,there is a dose-dependent relationship between compound Kushen injection and docetaxel,which can provide a reference in clinical medication.
作者
邢亚群
王迪生
汪庆飞
孙思雨
吴小祥
陈卫东
XING Ya-qun;WANG Di-sheng;WANG Qing-tei;SUN Si-yu;WU Xiao-xiang;CHEN Wei-dong(Department of Pharmacy,The Second Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233040;School of Pharmacy,Anhui U）ziversity of Chinese Medicine,Hefei Anhui 230031,China)
出处
《蚌埠医学院学报》
CAS
2018年第6期701-704,共4页
Journal of Bengbu Medical College
基金
国家自然科学基金面上项目(81473387)
