摘要
为优化吡虫啉降解菌固态发酵条件及确定适宜固态发酵基质,以菌株生物量为指标,筛选固体发酵培养基配方,通过单因素分析接种量、发酵温度、发酵时间对菌株生物量的影响,利用响应面分析法优化最佳发酵条件,同时进行菌制剂茶叶鲜叶吡虫啉降解小区试验分析。结果表明,不动杆菌BCL-1固体发酵适宜培养基配方为:草木灰65%+玉米粉8%+麸皮24%+葡萄糖2.3%+磷酸二氢钾0.5%+硫酸镁0.2%。适宜发酵时间72.80 h,接种量5.04%,发酵温度28.89℃,在此条件下,生物量可以达到9.537×108cfu/g。菌制剂可以有效降解茶叶鲜叶上的吡虫啉,施用12天后,茶叶鲜叶降解率达到82.0%。
The solid-fermentation condition of imidacloprid-degrading strain and its suitable solid-fermentation medium composition are optimized and determined. Taking biomass as the index, the effects ofthe fermentation conditions, such as medium component, inoculation, fermentation time and temperature, werediscussed by a single factor experiment, and the optimal fermentation condition of solid fermentation wasdetermined by using response surface analysis, and then the remediation effects of the microbial agents onimidaclorprid on tea leaves were studied. The results showed that the optimal fermentation condition was： plantash 65%, corn pink 8%, bran 24%, glucose 2.3%, KH2 PO30.5%, Mg SO40.2%; fermentation time 72.8 h,inoculum amount 5.04%, fermentation temperature 28.89℃. Under the condition, the number of biomassresearched 9.537×108 cfu/g. After 12 days, degradation rate of imidacloprid on the tea leaves reached 82.0%,showing an effective imidacloprid remediation potential.
作者
胡桂萍
石旭平
叶川
双巧云
黎小萍
杨广
施龙清
张建华
曹红妹
饶连英
欧阳雪灵
杨帆
Hu Guiping;Shi Xuping;Ye Chuan;Shuang Qiaoyun;Li Xiaoping;Yang Guang;Shi Longqing;Zhang Jianhua;Cao Hongmei;Rao Lianying;Ouyang Xueling;Yang Fang(I Jiangxi Sericulture and Tea Research Institute,Nanchang 33020;Institute of Applied Ecology,Fujian Agriculture and Forestry University,Fuzhou 350002;State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crop,Fuzhou 350002)
出处
《中国农学通报》
2018年第20期151-157,共7页
Chinese Agricultural Science Bulletin
基金
国家重点研究计划"农业土壤治理与改良关键技术的联合研发"(2016YFE0102100)
江西省重点研发计划项目"基于农药残留控制的茶叶安全生产关键技术联合研发与示范"(20161BBH80071)
闽台作物有害生物生态防控国家重点实验室开放课题基金(SKB2017011)
关键词
吡虫啉
降解菌
固体发酵
imidacloprid
degrading strain
solid fermentation
