摘要
目的探讨人口腔鳞状细胞癌(OSCC)组织中白细胞介素(IL-10)、信号传导与转录激活因子3(STAT3)与肿瘤相关巨噬细胞极化间的关系。方法采用免疫组织化学法检测IL-10、STAT3蛋白含量、CD68^+、CD163^+巨噬细胞阳性标记物在肿瘤组织中、正常组织中的表达水平并分析其与临床病理指标之间的相互关系。结果人口腔鳞状细胞癌组织中IL-10、STAT3蛋白的表达和CD163^+、CD68^+巨噬细胞浸润数均显著高于正常组(P<0.05)。STAT3、CD163^+、CD68^+在中低分化的表达高于高分化的表达(P<0.05)。IL-10、STAT3、CD163+在临床分期中Ⅲ+Ⅳ期患者较Ⅰ+Ⅱ期患者多(P均<0.05)。IL-10、STAT3、CD163+、CD68+在有淋巴结转移组较无淋巴结转移多(P均<0.05)。癌组织中IL-10、STAT3的表达与CD163+巨噬细胞浸润数之间呈正相关(r_1=0.347,r_2=0.397,P<0.05)。IL-10与STAT3的表达呈正相关(r=0.421,P<0.05)。结论口腔鳞癌中存在大量巨噬细胞浸润。M2型巨噬细胞与口腔鳞癌的发生、浸润、转移有关,其机制可能与肿瘤环境中IL-10及STAT3高表达有关,IL-10的表达上调激活STAT3可能使巨噬细胞更多向M2型转化,从而促进肿瘤的浸润与转移。
Objective To investigate the expressions of IL-10,STAT3,CD68 and CD163 in oral squamous cell carcinoma（OSCC）and its relationships between clinical characteristics and tumor-associated macrophage polarization.Methods By immunohistochemically method to detect the expressions of IL-10,STAT3,CD68^＋ and CD163^＋ in 42 primary OSCC tumor tissues and 15 samples with normal tissues as the control group.The correlation between macrophages distribution and clinical pathological features was explored.ResultsIt was found that theexpression of IL-10,STAT3,CD163^＋,CD68^＋ were significantly higher than normal group（P〈0.05）.STAT3,CD163^＋,CD68^＋ protein positive phenotype patients in the middle and poor differentiated group were higher than well differentiated group,IL-10,STAT3,CD163^＋ in the clinicalⅢ＋Ⅳstage patients were more than the clinicalⅠ＋Ⅱstagepatients.And the lymph node metastasis was significantly higher than that without lymph node metastasis in IL-10,STAT3,CD163^＋ and CD68（P〈0.05）,the number of M2 macrophages in OSCC was positively correlated with the expressions of IL-10,STAT3（r1=0.347,r2=0.397,P〈0.05）.IL-10 and STAT3 were positively correlated in OSCC（r=0.421,P〈0.05）.Conclusion M2 macrophage may be related with the occurrence,invasion and metastasis of OSCC,and IL-10,STAT3 suggesting a role for M2 macrophage polarization in this process and may participate in the development of OSCC.The upregulation of IL-10 activates STAT3 to make macrophages transform into M2 macrophages,so as to promote the infiltration and metastasis of tumor.
作者
叶丹
冯红超
宋宇峰
马竹君
Ye Dan1 , Feng Hongchao2 , Song Yufeng3 , Ma Zhujun2.(1. Guizhou Medical University, Guiyang 550004 , Guizhou, China; 2. Department of Oral and Maxillo facial Surgery, Guiyang Municipal Stomatological Hospital, Guiyang 550001 , Guizhou, China; 3. Food and Drug Administration Bureau in Guizhou Province, Guiyang 550004, Guizhou, China)
出处
《贵州医药》
CAS
2018年第6期646-649,769,共5页
Guizhou Medical Journal
基金
贵阳市卫生局科学技术计划项目[(2016)筑卫科技合同字第026号]
贵阳市卫生局科学技术计划项目[(2018)筑卫科技合同字第1-56号]
