摘要
目的从灵芝水提取生产过程中浓缩罐底部沉淀的浸膏状产物中分离制备灵芝酸。方法将灵芝水提取生产过程中浓缩罐底部沉淀的浸膏状产物溶于乙醇,超声,高速离心,得到上清液。采用Flash中压制备,粗分离获得3种样品,再通过prep高压制备,高效液相检测追踪。结果依次收集相同部位3种样品,经浓缩重结晶得白色晶体,纯度均达98%以上。获得3种结晶,分别经气相-质谱、红外光谱、1HNMR、和13CNMR等波谱手段,结合相关文献数据,确定白色结晶分别为灵芝酸C2、B和A。从副产物中提取得率分别为灵芝酸A:1.62%~3.00%;灵芝酸B:1.83%~2.59%;灵芝酸C2:1.03%~1.52%。结论利用富含灵芝酸的废弃副产物快速分离纯化制备出对照品,可为灵芝酸的药理研究和检测用对照品提供充足的来源。
Objective To isolate the ganoderic acids from the extract-like product precipitated from the bottom of the concentration tank during the water extraction and production process of Ganoderma lucidum（G.lucidum）.Methods The extract-like product precipitated at the bottom of the concentration tank in the production process of G.lucidum water extraction was dissolved in ethanol,sonicated,and centrifuged at high speed to obtain a supernatant.Three kinds of crude samples were obtained by using the preparation of Flash medium pressure,then prepared by Prep high pressure with detection tracking of HPLC.Results In turn,three samples of the same parts were collected,and white crystals were obtained by concentration and recrystallization,with the purity of over 98%.Three kinds of crystallization were identified respectively as Ganoderic acid C2,Ganoderic acid B and Ganoderic acid A by using GC-MS,IR,1 HNMR,and 13 CNMR and other spectral means,combined with relevant literature datas.The extraction rates from by-products were ganoderic acid A：1.62%-3.00%;ganoderic acid B：1.83%-2.59%;Ganoderic acid C2：1.03%-1.52%.Conclusion The use of rapid separation and purification of waste byproducts rich in ganoderic acid to prepare a reference substance provides a sufficient source for pharmacological research and detection of ganoderic acid.
作者
林冬梅
王赛贞
罗虹建
林占熺
林树钱
LIN Dongmei, WANG Saizhen, LUO Hongjian , LIN Zhanxi, LIN Shuqian.(National Engineering Research Center of JUNCAO Technology, Euzhou, Eujian 350001, China)
出处
《福建医药杂志》
CAS
2018年第3期135-138,共4页
Fujian Medical Journal
基金
国家菌草工程技术研究中心菌草生态产业协同创新攻关课题(JCXTGG08)
关键词
灵芝
灵芝提取副产物
全息快速纯化色谱仪
灵芝酸
结构鉴定
Ganoderma lucidum
processing by products of Ganoderma lucidum
holographic fast purification chromatograph
ganodcric acid
structural iclcntification
