摘要
目的:优选王瓜的微波-超声波联合提取工艺并考察其抗氧化活性。方法:以总三萜质量分数为指标,通过单因素试验和正交试验考察乙醇体积分数、提取时间、料液比、微波功率对王瓜提取工艺的影响;采用DPPH法、水杨酸法、ABTS法和FRAP法测定提取物的抗氧化能力。结果:最佳提取工艺条件为加50倍量90%乙醇于500 W微波-超声波联合提取6 min;王瓜提取物清除DPPH·、清除ABTS+·和Fe3+还原/抗氧化能力大于茶多酚但小于抗坏血酸,清除OH·能力均小于二者。结论:优选的提取工艺简便、快速,王瓜提取物具有一定的抗氧化性能。
Objective： To optimize ultrasonic-microwave synergistic extraction technology of total triterpenes from Trichosanthes cucumeroides and investigate its antioxidant activity.Method： With the content of total triterpenes as index,single-factor tests and orthogonal test were adopted to investigate effects of extraction time,ethanol concentration,solid-liquid ratio and microwave power on extraction technology.Antioxidant activity of its extract was extimated by DPPH assay,salicylicl acid assay,ABTS assay and FRAP assay.Result： The best extraction conditions for total triterpenes from T.cucumeroides were as follows： extracted 6 min with 50 times the amount of 90% ethanol,microwave power 500 W.Scavenging DPPH·,scavenging ABTS＋·and reducing Fe3＋ of extract from T.cucumeroides were higher than tea polyphenol and lower than Vc,but scavenging OH · was lower than theirs.Conclusion： This optimized technology was simple and rapid,extract of T.cucumeroides showed certain antioxidant activity.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第18期30-32,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中医药公共卫生专项国家基本药物所需中药原料资源调查和监测项目(财社[2011]76号)
中医药行业科研专项(20127002)
关键词
王瓜
总三萜
提取工艺
抗氧化活性
微波-超声波联合提取
Trichosanthes cucumeroides
total triterpenes
extraction technology
antioxidant activity
ultrasonic-microwave synergistic extraction
