摘要
背景:国内外研究表明,神经生长因子可明显促进烫伤创面愈合,但对于糖尿病烫伤创面局部神经生长因子联合用药的报道较少。目的:研究糖尿病大鼠深Ⅱ度烫伤创面局部应用神经生长因子-胰岛素凝胶后微血管密度及增殖细胞核抗原的变化。方法:将75只Wistar大鼠随机分为5组,正常对照组在大鼠背部制作深Ⅱ度烫伤模型,烫伤创面敷卡波姆980凝胶;其余4组均制作糖尿病模型,3 d后在大鼠背部制作深Ⅱ度烫伤模型,模型对照组烫伤创面敷卡波姆980凝胶,神经生长因子组、胰岛素组、联合组(此3组血糖正常)分别在烫伤创面敷含神经生长因子的卡波姆980凝胶、含胰岛素的卡波姆980凝胶及含神经生长因子-胰岛素的卡波姆980凝胶,各组每天换药1次,连续21 d。烫伤后3,7,11,15,21 d,检测各组大鼠创面愈合率及创面微血管密度及增殖细胞核抗原变化。结果与结论:(1)联合组烫伤不同时间点的创面愈合率、微血管密度及增殖细胞核抗原表达均高于其余4组(P<0.05);(2)苏木精-伊红染色显示,烫伤后1 d,各组表现为不同程度的组织结构破坏、血管扩张、炎性细胞浸润;烫伤后3 d后可见少量新生毛细血管,联合组最为明显;烫伤后7-11 d,各组毛细血管及炎性细胞逐渐增加;烫伤后15 d,联合组再上皮化明显,新生胶原纤维排列整齐,神经生长因子组、胰岛素组比正常对照组、模型对照组新生血管数量增多,但胶原纤维排列紊乱;烫伤后21 d,联合组创面被上皮细胞覆盖,胶原纤维排列整齐;其他组均有不同程度的上皮化,正常对照组、模型对照组不及神经生长因子组、胰岛素组;(3)结果表明,糖尿病大鼠深Ⅱ度烫伤创面局部应用神经生长因子-胰岛素凝胶,可增加微血管密度及增殖细胞核抗原的表达,利于创面愈合。
BACKGROUND： Researches have shown that nerve growth factor can promote wound healing, but there are few reports on the topical application of nerve growth factor combined with medication on diabetic scald wound.OBJECTIVE： To explore the changes of microvessel density and proliferating cell nuclear antigen expression after topical application of nerve growth factor-insulin gel on deep II scald wound in diabetic rats. METHODS： Seventy-five Wistar rats were randomly divided into five groups. Deep II scald models were made on the back of rats in normal control group followed by topical application of Carbopol 980. Diabetic models and deep II scald models were both made in the remaining rats with an interval of 3 days. Then, the rats in the latter four groups were subjected to topical application of Carbopol 980 alone, or Carbopol 980 containing nerve growth factor, insulin and combination of nerve growth factor and insulin, respectively. The wound in each rat was redressed once a day, for 21 consecutive days. Wound healing rate, microvessel density and proliferating cell nuclear antigen expression in rats were detected at 3, 7, 11, 15, 21 days after scald. RESULTS AND CONCLUSION： Wound healing rate, microvessel density and proliferating cell nuclear antigen expression were higher in the combination group than the other four groups at different time after scald（P〈0.05）. Results from hematoxylin-eosin staining showed that different structure damages, angiectasis and inflammatory cell infiltration were observed in different groups at 1 day after scald. A small amount of new capillaries were visible in each group, especially in the combination group at 3 days after scald. The number of capillary vessels and inflammatory cells increased gradually at 7-11 days after scald. Marked re-epithelialization and regular arrangement of collagen fibers were observed in the combination group at 15 days after scald. Meanwhile, compared with the normal control group and model group, the number of new vessels was increased
作者
徐海涛
王敏
向喜娟
Xu Hai-tao;Wang Min;Xiang Xi-juan(Department of Orthopedics,Yongchuan Hospital of Chongqing Medical University,Chongqing 402160,China;Department of Burn,Central Hospital of Zibo,Zibo 255000,Shandong Province,China;Department of Ophthalmology,Yongchuan District Hospital of Traditional Chinese Medicine,Chongqing 402160,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第22期3506-3512,共7页
Chinese Journal of Tissue Engineering Research
基金
重庆医科大学附属永川医院资助项目(YJLCX201540)~~
