摘要
花生耐盐遗传位点的挖掘可为耐盐遗传机制研究奠定基础,可为耐盐品种培育提供基因资源。以花育28号和P76构建的RIL群体为材料,基于该群体构建的高密度遗传图谱,结合2个年份盐胁迫下的表型数据,应用Ici Mapping软件对花生盐胁迫下相关性状进行QTL分析。检测到13个盐胁迫下表型绝对值相关的QTL,分布于10个连锁群上,其中株高绝对值相关的QTL 3个、主根长绝对值相关的QTL 2个、茎叶质量绝对值相关的QTL 6个、根质量绝对值相关的QTL 2个。检测到6个盐胁迫下表型相对值相关的QTL,其中株高相对值相关的QTL 3个、主根长相对值相关的QTL 2个、茎叶鲜质量相对值相关的QTL 1个,分布于5个连锁群上。对比发现,B02染色体上Marker774175标记附近检测到4个QTL:q Smsh-HP-B02.1、q Swfp-HP-B02.1、q Rmsh-HP-B02.1、q Rwfp-HP-B02.1,分别控制盐胁迫下的株高和茎叶鲜质量的绝对值和相对值,其增效等位基因均来源于花育28号。结果对于进一步挖掘花生耐盐QTL具有重要意义。
The identification of salt tolerant genetic loci in peanut can provide basis for the molecular mechanism of salt tolerance,and gene sources for improving salt tolerant cultivars. Based on a high-dense genetic map being constructed by using a RIL population derived from the cross between Huayu 28 and P76,we performed QTL analysis for vegetative traits of peanut under salt stress by software IciMapping. As a result,13 QTLs related to absolute value of traits on salt stress on ten linkage groups were detected. 3,2,6 and 2 of 13 QTLs controlled absolute value of plant height,root length,shoot fresh weight and root weight,respectively. 6 QTLs related to relative value of traits on salt stress on five linkage groups were detected. 3,2 and 1 of 6 QTLs controlled relative value of plant height,root length and shoot fresh weight,respectively. 4 QTLs detected in B02 were linked to Marker774175,which were qSmsh-HP-B02.1 , qSwfp-HP-B02.1 , qRmsh-HP-B02.1 , qRwfp-HP-B02.1 related to absolute value and relative value of plant height and stem fresh weight on salt stress,respectively. Synergistic genes of the 4 QTLs derived from Huayu 28. The results provided important information for further exploration of salt tolerant QTL in peanut .
作者
苗华荣
杨伟强
胡晓辉
张胜忠
崔凤高
陈静
MIAO Huarong;YANG Weiqiang;HU Xiaohui;ZHANG Shengzhong;CUI Fenggao;CHEN Jing(Shandong Peanut Research Institute,Qingdao 266100,China)
出处
《华北农学报》
CSCD
北大核心
2018年第3期113-118,共6页
Acta Agriculturae Boreali-Sinica
基金
山东省农业良种工程项目(2017LZGC003)
山东省农业科学院创新工程项目(CXGC2016B02
CXGC2018E2)
山东省重点研发计划项目(2016ZDJS10A02)
山东省农业科学院重大成果培育项目
山东省农业科学院英才计划
山东省渤海粮仓科技示范工程项目(2017BHLC019)
关键词
花生
幼苗期
盐胁迫
QTL
Peanut；Seedling stage；Salt stress；QTL
