摘要
【目的】探究花生ASR基因家族特性及其在干旱和盐胁迫响应中的作用,为培育花生抗旱抗盐新品种提供潜在基因位点。【方法】通过生物信息学方法在全基因组水平鉴定花生ASR家族并分析其基本特性,借助转录组数据分析其在200 mmol/L NaCl及模拟干旱PEG处理后的表达变化。【结果】(1)鉴定到7个花生ASR基因,pI为5.34~6.98,蛋白脂肪系数为23.77~56.84,GRAVY值均为负值,表明这7个蛋白均是亲水性蛋白;(2)AhASR3与AhASR7基因表达模式相似,转录水平较高,基因结构及蛋白结构域和保守基序的位置和数量较相似,motif 5、6、9仅存在于AhASR3与AhASR7蛋白中;(3)AhASR1、AhASR5及AhASR2的启动子区域有干旱诱导MYB转录因子的结合位点,发现AhASR1、AhASR2及AhASR4的启动子区有ABA响应元件;(4)AhASR2、AhASR3及AhASR7在200 mmol/L NaCl处理后根部出现较明显转录上调;(5)AhASR1、AhASR3、AhASR4及AhASR7分别在PEG处理4 h和8 h后,转录水平出现2倍以上上调。【结论】花生ASR家族蛋白均为亲水性蛋白,各成员之间既有保守结构域也有特异性序列,大部分成员启动子区都具有干旱响应相关元件,并受到盐处理以及模拟干旱处理的诱导表达,可作为耐盐耐旱花生品种培育的重要候选基因。
[Objective]The aim of this study is to explore the characteristics of peanut ASR gene family and their roles in response to drought and salt stress,and to provide potential gene loci for the breeding of drought-and salt-resistant peanut varieties.[Methods]The ASR gene family of peanut was identified and its characteristics were analyzed by bioinformatics method at the genome level.Transcriptome data was used to analyze expression changes after 200 mmol/L NaCl and PEG treatment.[Results](1)Seven ASR genes were identified,with pI value ranging from 5.34 to 6.98.The aliphatic index was between 23.77-56.84 and the GRAVY value was negative,indicating that these proteins were hydrophilic.(2)The expression pattern of AhASR3 and AhASR7 genes was similar,with high transcription level,similar gene structure,protein structure domain,and the number of conserved motifs,and motifs 5,6,and 9 were only found in AhASR3 and AhASR7 proteins.(3)The promoter regions of AhASR1,AhASR5,and AhASR2 contained drought-induced MYB transcription factor binding sites,and the promoter regions of AhASR1,AhASR2,and AhASR4 contained ABA response elements.(4)The transcription of AhASR2,AhASR3,and AhASR7 was up-regulated in roots at 200 mmol/L NaCl.(5)The transcription levels of AhASR1,AhASR3,AhASR4,and AhASR7 were up-regulated more than two-fold after PEG treatment for 4 h and 8 h.[Conclusion]ASR proteins in peanut are hydrophilic,and there are both conserved domains and specific sequences among ASR members.Most of the members have drought response elements in the promoter region and are induced by salt treatment and PEG treatment,which can be used as important candidate genes for the cultivation of salt-and drought-tolerant peanut varieties.
作者
宁东贤
杨秀丽
程麦凤
刘博
牛芮
张泽
NING Dongxian;YANG Xiuli;CHENG Maifeng;LIU Bo;NIU Rui;ZHANG Ze(Institute of Wheat Research,Shanxi Agricultural University,Linfen,Shanxi 041000,China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2024年第7期1113-1121,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
山西省科技重大专项计划揭榜挂帅项目——重大基础前沿与民生公益类项目(202201140601025-4-04)
山西农业大学生物育种工程——高产优质早熟花生新品种选育项目(YZGC018)
山西农业大学“引进人才科研启动工程”项目(2023BQ113)。
关键词
花生
干旱胁迫
盐胁迫
ASR基因
peanut(Arachis hypogaea L.)
drought stress
salt stress
ASR gene
