摘要
[目的]研究细茎石斛多糖的最佳提取条件及其抗氧化活性。[方法]通过单因素试验和响应面分析,研究细茎石斛多糖(DMP)的最佳提取条件。通过体外试验评价细茎石斛多糖的体外抗氧化活性。[结果]在料液比为1∶53,时间为3 h,温度为83℃的提取条件下,DMP最高产量可达185 mg/g。体外抗氧化活性研究结果表明,DMP对超氧阴离子自由基、ABTS自由基、羟基自由基具有明显的清除作用,其中对ABTS自由基清除能力与维生素C相当;DMP对DPPH自由基的清除能力和还原能力效果比较适中。[结论]该研究揭示细茎石斛多糖可以作为一种天然抗氧化剂,为基于多糖的药物及保健食品的开发提供新思路。
[Objective] The research aimed to study the optimal extraction conditions and antioxidant activity of polysaccharide from Dendrobium monliforme.[Method] Through single-factor tests and response surface analysis,the optimal conditions for extraction of polysaccharide from Dendrobium monliforme were studied. The antioxidant activity of polysaccharide from Dendrobium monliforme was evaluated in vitro.[Result]The maximum yield obtained 185 mg/g at solid-liquid ratio 1 ∶53,time 3 hours and temperature 83 ℃.The results of in vitro antioxidant activity showed that DMP had obvious scavenging effects of superoxide anion radical,ABTS radical,hydroxyl radical,while moderate scavenging effects of DPPH radical. In addition,ABTS radical scavenging activity of DMP was as stronger as vitamin C. [Conclusion]This polysaccharide could be developed as a new source of natural antioxidants,polysaccharide-based drugs or food ingredients with specific health improving functions.
作者
Muhammad Naeem Asghar
Muhammad Naeem Asghar(School of Food Science and Engineering,Hefei University of Technology,Hefei,Anhui 23000)
出处
《安徽农业科学》
CAS
2018年第20期1-5,共5页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(20872024)
关键词
细茎石斛
多醣
最佳提取条件
抗氧化活性
Dendrobium moniliforme
Polysaccharide
Optimal extraction conditions
Antioxidant activity
