摘要
以水提醇沉法制备川芎粗多糖(1)。首次报道了1与氯磺酸-吡啶经亲核取代反应半合成硫酸酯化川芎粗多糖(2)的方法,其结构经UV和IR表征。用氯化钡-明胶比浊法测定2的取代度。考察了1和2对邻苯三酚自氧化反应产生的超氧阴离子(O2-·)和1,1-二苯基-2-苦基肼(DPPH)体外清除自由基能力。结果表明:在测定浓度范围内,1和2对O2-·清除能力极弱,但对DPPH自由基有一定的清除作用。
Rhizoma chuanxiong crude polysaccharides（1） was obtained by water extraction and alcohol precipitation method.The sulfated Rhizoma chuanxiong polysaccharides（2） were prepared by nucleophilic substitution of 1 with chlorosulfonic acid-pyridine.The synthetic method was first time reported.The structures were confirmed by UV and IR.The degree of substitution of 2 were measured by the barium chloride-gelation turbidimetry.Clearance rate in vitro of 1 and 2 on superoxide anion（O2-·） and 1,1-diphenyl-2-picrylhydrazyl）（DPPH） were investigated.The results showed that 1 and 2 exhibited very weak cleaning ability on O2-·,had positive cleaning ability on DPPH free radical.
出处
《合成化学》
CAS
CSCD
北大核心
2013年第4期428-431,共4页
Chinese Journal of Synthetic Chemistry
基金
四川省应用基础研究项目(2009JY0113)
关键词
川芎粗多糖
硫酸酯化
半合成
抗氧化活性
Rhizoma chuanxiong crude polysaccharide
sulfated
semisynthesis
anti-oxidative activity
