摘要
目的探讨BMP2信号通路在特发性高钙尿大鼠肾结石形成中的调控作用。方法取20只遗传性高钙尿结石大鼠(GHS组)和20只体质量、月龄配对的SD大鼠(对照组),适用性喂养1周后处死,取出肾脏组织。用Von Kossa钙染色法检测肾脏组织钙盐沉积情况,Western Blot法检测BMP2、MSX2、RUNX2和Osx蛋白表达,RT-PCR检测BMP2、MSX2、RUNX2和Osx mRNA表达。结果 GHS组大鼠肾间质中有明显的钙盐沉积,对照组大鼠肾间质无钙盐沉积。RT-PCR检测结果显示,GHS大鼠BMP2、MSX2、RUNX2和Osx mRNA表达水平明显高于对照组,差异有统计学意义(P<0.05)。Western Blot检测结果显示,GHS大鼠肾脏组织BMP2、MSX2、RUNX2和Osx蛋白明显高于对照组,差异有统计学意义(P<0.05)。结论 GHS大鼠肾间质有明显钙化情况,高钙尿可能通过激活BMP2信号通路调控肾结石的形成。
Objective To explore the modulation role of BMP2 signaling pathway in kidney calcification in idiopathic hypercalciuria rats.Methods A total of 20 genetic hypercalciuric stone-forming rats（GHS group）and 20 of similar elder and weight SD rats（control group）were selected,the rats were sacrificed after 1 week of adaptive feeding.Fresh kidney tissue was collected,the calcification in tissue was measured by Von Kossa assay,the expression of BMP2,MSX2,RUNX2 and Osx protein was detected by Western Blot,the expression of mRNA were measured by RT-PCR.Results There existed apparent renal interstitial calcification in GHS rats while there was no calcification in SD rats.The results of RT-PCR showed that the expression of BMP2,MSX2,RUNX2 and Osx protein in the GHS group was higher than in the control group,the difference was statistically significant（P0.05）.In GHS rats,the BMP2,MSX2,RUNX2 and Osx mRNA expression was significantly increased compared with normal rats,the difference was statistically significant（P0.05）.Conclusion There is apparent renal interstitial calcification in GHS rats,hypercalcification may through the activation of BMP2 signaling pathway to the formation of kidney stones.
作者
白栩搏
BAI Xubo(Department of Urology,the General Hospital of Jizhong Energy Xingtai Mining Group Corporation,Xingtai 054000,China)
出处
《西北药学杂志》
CAS
2018年第4期503-506,共4页
Northwest Pharmaceutical Journal
关键词
特发性高钙尿
肾结石
骨形成蛋白
信号通路
idiopathic hypercalciuria
renal calculus
bone morphoenetic protein
signaling pathway
