摘要
目的分析吗啡与布托啡诺对肺腺癌细胞A549侵袭与迁移的影响及可能的作用机制。方法人肺腺癌A549细胞随机分为吗啡组与布托啡诺组。每组均分别设置吗啡、布托啡诺与拮抗剂不同浓度。采用免疫组化法、Transwell法、细胞划痕法评估吗啡与布托啡诺及其对应的拮抗剂对肺腺癌细胞A549侵袭与迁移及相关蛋白表达的影响及作用机制。结果随着布托啡诺剂量的增加,A549细胞侵袭数明显减少,而随着吗啡剂量的增加,A549细胞侵袭数明显增加(P<0.05);随着布托啡诺剂量的增加及时间延长,A549细胞迁移变化不明显,而随着吗啡剂量的增加A549细胞迁移数明显增加(P<0.05);Nor-Binaltorphimine(Nor-BNI)剂量为0.1μmol/L时,A549细胞侵袭数明显增加。与单纯布托啡诺组比较,加入Nor-BNI后,每组对应剂量的A549细胞侵袭数明显增加。与单纯吗啡组比较,随着甲基纳曲酮剂量的增加,A549细胞侵袭数明显减少。10μmol/L布托啡诺作用肺腺癌A549细胞24 h后,相关蛋白表达下调,而吗啡作用后表达上调(P<0.05)。结论吗啡可促进肺腺癌细胞A549的迁移与侵袭,并上调MMP-2、MMP-9、Fascin蛋白表达,而布托啡诺则抑制肺腺癌细胞A549的迁移与侵袭,并下调MMP-2、MMP-9、Fascin蛋白表达。
Objective To investigate the effect of morphine and butorphanol on the invasion and migration of lung adenocarcinoma A549 cells and its possible mechanism. Methods Human lung adenocarcinoma A549 cells were randomly divided into morphine group and butorphanol group. Each group was given different concentrations of morphine,butorphanol and antagonists. The influence of morphine,butorphanol and their corresponding antagonists on the invasion and migration of lung adenocarcinoma A549 cells was evaluated by immunohistochemistry,Transwell assay and cell scratch assay. Results With the increase of butorphanol dose,the number of invasiveness of A549 cells decreased significantly,while with the increase of morphine dose,the invasion number of A549 cells increased significantly（ P〈0. 05）. With the increase of dose of butorphanol and the prolongation of time,the migration of A549 cells was not obvious. However,the migration of A549 cells was significantly increased with the increase of morphine dose（ P〈0. 05）. When the dose of Nor-binaltorphimin e（ Nor-BNI） was 0. 1 μmol/L,the invasion of A549 cells was significantly increased. Compared with simple butorphanol group,the invasion number of A549 cells in each group significantly increased after addition of Nor-BNI. Compared with simple morphine group,with the increase of methylnaltrexone dose,the invasion number of A549 cells decreased obviously. After treated with 10 μmol/L butorphanol for 5 days,A549 cells were down-regulated,while up-regulated after morphine treatment（ P〈0. 05）. Conclusion Morphine can promote the migration and invasion of lung adenocarcinoma A549 cells and up-regulate the expression of MMP-2,MMP-9 and Fascin protein,while butorphanol inhibits the migration and invasion of lung adenocarcinoma A549 cells and down-regulates the expression of MMP-2,MMP-9,Fascin protein expression.
作者
李彬
薛昀
Li Bin;Xue Yun(School of Clinical Medicine,Southwest Medical University,Luzhou 646000;Dept of Anesthesiology,Fuling Central Hospital,Chongqing 408000)
出处
《安徽医科大学学报》
CAS
北大核心
2018年第9期1392-1397,共6页
Acta Universitatis Medicinalis Anhui
基金
重庆市科技计划项目(编号:cstc2013jcyj A10023)
