摘要
目的观察不同浓度罗哌卡因对乳腺癌MCF-7细胞增殖和细胞周期的影响并探讨其机制。方法人乳腺癌细胞MCF-7接种于培养板培养24h,随机分为四组:对照组(C组)、罗哌卡因100μg/ml组(R1组)、罗哌卡因200μg/ml组(R2组)和罗哌卡因400μg/ml组(R3组)处理乳腺癌MCF-7细胞48h后,检测其细胞增殖能力(细胞活力)和细胞周期。检测R3组作用于MCF-7细胞48h后TCF-4、beta-catenin的蛋白表达水平。结果 R2、R3组MCF-7细胞活力明显低于C组(P<0.05);R1、R2、R3组MCF-7细胞G0/G1期细胞明显少于C组,S期和G2/M期细胞明显多于C组(P<0.05);R3组TCF-4和beta-catenin蛋白表达水平明显低于C组(P<0.05)。结论罗哌卡因可能通过下调TCF-4和beta-catenin蛋白表达水平抑制人乳腺癌MCF-7细胞增殖。
Objective To observe the different behavior of proliferation and cell cycle of MCF-7cells when exposured to ropivacaine of different concentrations and further explore its underlying mechanism.Methods Human breast cancer cells MCF-7were inoculated into culture medium for 24 h,then were randomly divided into four groups：Control group（group C）,Ropivacaine 100μg/ml group（group R1）,Ropivacaine 200μg/ml group（group R2）,Ropivacaine 400μg/ml group（group R3）.We medicated each group and incubated for 48 h,then detected the cell proliferation and cell cycle immediately.The level of protein TCF-4and beta-catein of groups R3 and C were measured at the same time.Results MCF-7cell viability of groups R2 and R3was significantly lowed（P〈0.05）,MCF-7cell viability of group R1 had no significant difference when compared to group C.G0/G1 phase cells of groups R1,R2 and R3were significantly less than those of group C,S phase cells of groups R1,R2 and R3were significantly more than group C,G2/M phase cells of groups R1,R2 and R3were significantly more than group C（P〈0.05）.The expression level of TCF-4and beta-catenin in group R3 was significantly lower than that in group C（P〈0.05）.Conclusion Ropivacaine inhibits the proliferation of breast cancer cells MCF-7by down-regulating TCF-4and beta-cateni.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2016年第7期680-683,共4页
Journal of Clinical Anesthesiology
关键词
罗哌卡因
乳腺癌
增殖
细胞周期
Ropivacaine
Breast cancer
Proliferation
Cell cycle
