摘要
[目的]建立HPLC-DAD同时检测饲用植物提取物中非法添加喹乙醇、乙酰甲喹的含量。[方法]采用ZORBAX Eclipse XDB-C18(4.6 mm×150 mm,5μm)分离,以甲醇-磷酸溶液(0.025 mol/L,三乙胺调节p H=6.0)20∶80(V/V)为流动相,流速1.0 m L/min,检测波长260 nm,柱温40℃,采用二极管阵列紫外检测器(DAD)检测,外标法定量,供试品相应色谱峰的保留时间和光谱图与对照品的进行比较定性。[结果]喹乙醇、乙酰甲喹分离度良好,浓度分别在5.03~201.32、5.04~201.38μg/m L与峰面积呈良好的线性关系,平均回收率分别为99.76%、99.28%,RSD分别为0.97%、0.78%,检测限为5 mg/kg,定量限为10 mg/kg。[结论]该方法精密度高、重复性好、操作简便,适用于同时检测饲用植物提取物中非法添加喹乙醇、乙酰甲喹含量。
[ Objective ] The research aimed to establish HPLC-DAD to simuhaneously detect the illegal addition of olaquindox and aeetoquine in forage plant extraets. [ Methodl The samples wine separated by ZORBAX Eelipse XDB-C18 （ 4.6 mmx 150 mm,5 μm）, using aeetonitrile and 0.025 mol/L phosphorie aeid solution（ Regulated pH = 6.0 with triethylamine） at 20：80（WV） volmne ratio as mobile phases with flow rate 1.0 mL/min ,deteeted by 260 nm, deteeted at eolmnn temperatme 40 ℃, deteeted by Diode array ultraviolet deteetor（DAD）. Retention time and speetrogram of ehmmatographie peaks were eompared and qualitatively analyzed between samples and standards with external standard quantitative analysis. [ Resultl The eoneentration of 5.03-201.32 and 5.04-201.38 μg/mL showed a good linear lelationship with the peak are- a. The average leeoveries wele 99.76% and 99.28%, lespeetively. The RSD was 0.97% and 0.78% lespeetively, the deteetion limit was 5 mg/kg, and the limit of quantifleation was 10 mg/kg. [ ConelusionlThe method has high precision, good repeatability and simple operation, and is suitable for simuhaneously deteeting the illegal addition of olaquindox and aeetoquine in teed plant extraets.
作者
吴昊
刘发全
宋亚伟
丁在亮
WU Hao;LIU Fa-quan;SONG Ya-wei(Veterinary Drag and Feed Supervision Institute of Anhui Province,Hefei,Anhui 230091)
出处
《安徽农业科学》
CAS
2018年第24期152-154,194,共4页
Journal of Anhui Agricultural Sciences
