摘要
[目的]通过采用实验手段人为调控森林样地凋落物量,改变土壤碳输入,以探究土壤酶活性与碳源输入之间的关系。[方法]以河南宝天曼自然保护区内不同年龄序列(林龄大约为40 a、80 a和大于160 a)的锐齿栎天然林为研究对象,在样地内进行对照(CK)、凋落物加倍(LA)、凋落物去除(LR)3种处理,分别测定了土壤理化性状、微生物生物量、并且采用底物诱导法对氧化酶(酚氧化酶和过氧化物酶)和水解酶(β-葡萄糖苷酶和N-乙酰-β葡萄糖苷酶)的活性进行测定。[结果]林龄仅对N-乙酰-β葡萄糖苷酶有显著影响,其酶活性随着林龄的增加而降低。碳输入的改变对氧化酶无显著差异,但β-葡萄糖苷酶活性随凋落物量的增加而提高,呈现加倍>对照>去凋的趋势。微生物碳氮比(表征微生物结构)变化规律与专一酶活性(微生物功能)变化规律之间无严格对应关系。[结论]碳输入量的改变可以通过影响土壤环境因子和养分输入,从而对土壤酶活性产生影响,但对不同酶活性的影响程度不同,同时林分年龄也是影响土壤酶活性的一个重要指标。
[ Objective] To determine the relationship between soil enzyme activities and carbon inputs by experi- mentally ahering the quantity of forest floor litter. [ Method ] In an age sequence of natural Quercus aliena vat. acuteserrata forests （ stand age approximately 40 years, 80 years, and 〉 160 years） in the Baotianman Nature Re- serve, He＇ nan Province, three treatments were applied, including doubling of litter （LA）, litter ~moval （LR） and the control （CK）. Measurements were made on soil physiochemical properties, microbial biomass, and activi- ties of oxidative enzymes （ phenol oxidase and peroxidase） and hydrolytic enzymes（ β-glucosidase and [3-1,4-N-acetylglucosaminidase）. [Result] The stand age affected only the β-1,4-N-acetylglucosaminidase; its activity de- creased with the increasing stand age. The litter treatments had no significant effect on oxidative enzymes, but the activity of β-glucosidase increased with the increasing litter inputs, ranking in the order of LA 〉 CK 〉 LR. The change pattern of microbial C/N ratio （characterization of microbial structure） were not consistent with that of spe- cific enzyme activity （microbial function）. [Conclusion] The changes in the quantity of carbon inputs can affect soil enzyme activities by modifying soil environmental factors and nutrient inputs, but the effects vary among en- zymes. Forest stand age is also a factor affecting soil enzyme activities.
作者
李程
尤业明
唐佐芯
孙建新
LI Cheng;YOU Ye-ming;TANG Zuo-xin;SUNdian-xtn(College of Forestry,Beijing Forestry University,Beijing 100083,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Forestry College,Guangxi University,Nanning 530004,Guangxi,China)
出处
《林业科学研究》
CSCD
北大核心
2018年第4期23-30,共8页
Forest Research
基金
国家自然科学基金资助项目“亚热带一温带过渡区森林土壤有机碳固持机制及其微环境制约”(项目编号：31470623)
关键词
碳输入
土壤酶活性
土壤微生物群落
年龄序列
宝天曼
carbon input
soil enzyme activity
soil microbial conmmnity
age sequence
Baotianman Nature Re-serve
