摘要
【背景】拉鲁湿地是我国海拔最高、面积最大的城市天然湿地。开展拉鲁湿地微生物群落结构分析,可为青藏高原生物资源的开发利用和保护提供理论依据,并为湿地生态系统的微生物多样性研究奠定基础。【目的】开展拉鲁湿地水体丝状真菌的多样性研究,探究影响该环境丝状真菌群落分布特征的主要理化因子。【方法】从拉鲁湿地11个水样中分离丝状真菌,应用nr DNA ITS序列分析,并结合经典分类学方法对获得的菌株进行鉴定,运用SPSS和CANOCO软件分析丝状真菌群落结构及其与环境因子的关系。【结果】从拉鲁湿地水体分离的丝状真菌菌株分属于6属13种。拉鲁湿地冬季水体丝状真菌优势属为Mucor、Cladosporium和Galactomyces,优势种为M.hiemalis、M.racemosus和G.geotrichum。总氮与拉鲁湿地水体丝状真菌数量呈显著负相关(P<0.05),总氮和总磷对于丝状真菌的分布具有较明显的影响,这2种理化因子与M.racemosus的多度呈正相关性,而与M.hiemalis呈较强的负相关性。【结论】环境因子是影响高原湿地生态系统微生物群落结构的主要因素之一,探讨西藏典型脆弱生态环境中微生物多样性与环境因子的关系具有重要意义。
[Background] Lhalu Wetland was the highest and largest urban natural wetland in China. Studying on microbial community structure in Lhalu Wetland could provide theoretical basis for exploitation and protection of biological resources in Qinghai-Tibet plateau, and lay foundation for the study of microbial diversity in wetland ecosystem. [Objective] To investigate diversity of mycelial fungus and explore the main environmental factors which influenced the community structure of filamentous fungi in Lhalu Wetland. [Methods] Fungi were isolated from 11 water samples, and identification of fungi were completed using the sequence analysis of nr DNA ITS, combining with traditional classified method. SPSS and CANOCO were used to examine correlations between filamentous fungi diversity and environmental factors. [Results] Mycelial fungi isolated from Lhalu Wetland were belonging to 6 genera and 13 species. Mucor, Cladosporium, Galactomyces were the dominate genera in the wetland. M. hiemalis, M. racemosus and G. geotrichum were the dominate species. Statistical analysis indicated that TN has significantly negative correlation with the counts of filamentous fungi at the Lhalu Wetland（P〈0.05）, moreover, TN and TP had significant effect on distribution of the fungi, which positively correlated with M. racemosus but passively correlated with M. hiemalis. [Conclusion] Environmental variable has been one of the major factors that influencing wetland microorganism community structure, therefore, it was crucial concerning to study the relationship between environmental factors and diversity of microbe in Tibet.
作者
郭小芳
德吉
龙琦炜
白斌锦
王豪杰
曹亚璞
GUO Xiao-Fang;DE Ji;LONG Qi-Wei;BAI Bin-Jin;WANG Hao-Jie;CAO Ya-Pu(School of Science,Tibet University,Lhasa,Tibet 850000,Chin)
出处
《微生物学通报》
CAS
CSCD
北大核心
2018年第8期1661-1675,共15页
Microbiology China
基金
国家重点研发计划项目(2016YFC0502000)
西藏自治区自然科学基金(2016ZR-15-11)
2016年度西藏大学珠峰学者人才发展支持计划(藏大字[2016]141号)~~
关键词
青藏高原
湿地
环境因子
真菌
多样性
Qinghai-Tibet Plateau
Wetland
Environmental factors
Fungi
Diversity
