摘要
通过探究发酵豆粕替代鱼粉对黄姑鱼幼鱼肌肉中氨基酸组成、肌肉和肝脏中IGF-I基因相对表达量及肝脏组织形态结构的影响,以确定黄姑鱼幼鱼饲料中发酵豆粕替代鱼粉的适宜比例。试验以发酵豆粕、豆粕、鱼粉和小麦蛋白粉为蛋白质源,鱼油、豆油和大豆卵磷脂为主要脂肪源,配制含45%鱼粉的基础饲料。以发酵豆粕替代基础饲料中0%(FSM0组)、10%(FSM10组)、20%(FSM20组)、30%(FSM30组)、40%(FSM40组)、50%(FSM50组)的鱼粉,共配制6组等氮(蛋白质水平为50%)等脂(脂肪水平为12%)的饲料。各实验组(除对照组)均添加适量的蛋氨酸和赖氨酸,使得各组蛋氨酸和赖氨酸水平一致。试验选取360尾平均体重为(31.24±0.02)g的黄姑鱼幼鱼,每组设置3个重复,每个重复为20尾黄姑鱼,饲养于500 L水桶中。在水温(28±2)℃,盐度27~30的条件下养殖8周。实验结果表明:缬氨酸和蛋氨酸均无显著性差异(P>0.05),其余氨基酸均随着发酵豆粕替代水平的提高而呈现增高的趋势;黄姑鱼肌肉中IGF-I表达量呈先上升后下降的趋势,在FSM30组时显著最高;黄姑鱼肝脏中FSM50组IGF-I表达量显著低于对照组;通过肝脏组织学观察发现,随着发酵豆粕替代水平的增加,肝脏细胞受损严重,FSM50组肝细胞轮廓模糊,部分肝细胞核甚至已经消失不见。结果分析表示,本实验条件下,发酵豆粕替代鱼粉可以有效提高黄姑鱼鱼体中氨基酸的积累(除缬氨酸和蛋氨酸),替代量在20%-30%为宜否则会降低肝脏和肌肉中IGF-I的表达量并造成肝脏损伤。
The purpose of this study was to explore the effect of replacing fish meal with fermented soy-bean meal on the relative expression of IGF-I gene in the muscle and liver and the structure of liver tissue of juvenile Nibea albiflora. A 45% fish meal basal diet was formulated using fermented soybean meal, soybeanmeal, fish meal and wheat protein powder as main protein sources, and fish oil soybean oil and soybeanlecithin as main lipid sources. Six isonitrogenous （protein level was 50%） and isolipidic （lipid level was 12%）experimental diets were formulated by replacing 0 （FSM0, as control group）, 10% （FSM10 group）, 20% （FSM20group）, 30% （FSM30 group）, 40% （FSM40 group） and 50% （FSM50 group） fish meal with FSM on the basis ofthe basal diet, respectively. Appropriate amount of Methionine and lysine were added in each group （except thecontrol group） to ensure the levels of methionine and lysine in each group were consistent. 360 fish with theaverage body weight of（31.24+-0.02） g were randomly divided into six groups with 3 repetitions in each group.Each group has 20 juvenile N. albiflora, which were kept ina 500 L tank. An 8-week growth experiment wascarried out during the condition of water temperature（28+-2）C and salinity 27 to 30. The analysis results ofamino acid （AA） in muscle indicates that there were no significant difference between valine and methionine,other amino acids （except valine and methionine） were positively correlated with the replacement level. TheIGF-I gene expression in N. albiflora liver fed FSM 50 diet was significantly lower than that in control group.The IGF-I gene expression in N. albiflora muscle raised at first and goes down latter with the increase of thereplacement, and reached it’ s peak when the substitution is 30%; Histopathological study showed liver cellswere damaged severely with the increase of the replacement. In the group FSM50, the outlines of liver cells areonly barely visible and loss of nuclei. Overall, in this test, replac
作者
胡沈玉
王立改
楼宝
曾文繁
谭朋
詹炜
陈睿毅
刘峰
谢庆平
徐冬冬
HU Shen-yu;WANG Li-gai;LOU Bao(Marine and Fishery Institute of Zhejiang Ocean University,Marine Fisheries Research Institute of ZhejiangProvince,Key Lab of Mariculture and Enhancement of Zhejiang Province,Zhoushan 316021,China)
出处
《浙江海洋大学学报（自然科学版）》
CAS
北大核心
2018年第3期196-202,共7页
Journal of Zhejiang Ocean University：Natural Science
关键词
黄姑鱼
发酵豆粕
鱼粉
氨基酸
IGF-I
组织形态结构
juvenile Nibea albiflora
fermented soybean meal
fishmeal
amino acids
IGF-I
organization-al structure
