摘要
为对大皂角与猪牙皂的形态结构与成分进行分析,采用游标卡尺等工具测量荚果外部形态和结构指标,利用超声波提取法和HPLC法测定粗皂苷和刺囊酸的含量。结果表明,大皂角与猪牙皂大、小果的荚果长、宽、厚、质量、长宽比差异都达到极显著水平,大皂角荚果长宽积为最大,与猪牙皂小果差异极显著,与猪牙皂大果差异显著;大皂角与猪牙皂大、小果孔纹细胞壁厚且明显,可见气孔,但大皂角与猪牙皂大果具有栅状细胞,具有种子且石细胞数众多,与猪牙皂小果显著差异。大皂角、猪牙皂小果、猪牙皂大果的粗皂苷含量分别为37.40%、46.70%、35.15%,刺囊酸含量分别为0.37%、0.11%、0.27%,大皂角与猪牙皂大小果的粗皂苷和刺囊酸含量之间均呈极显著差异。综上所述,大皂角与猪牙皂形态结构及成分存在极显著差异。
In order to compare and analyze the structure and composition of Gleditsia sinensis Lain. and fructus gleditsiae abnormalis, external morphology and structure of Gleditsia sinensis Lain. , small and big fruit of fructus gleditsiae abnormalis were measured by vernier caliper, and contents of crude saponins and echinocyctic acid were measured by ultrasonic and HPLC methods. The results showed that the difference of the pod length,width, thickness, weight,length width ratio between Gleditsia sinensis Lain. and big and small fruit of fructus gleditsiae abnormali was extremely significant. Among them the pod length and width product of Gleditsia sinensis Lain. was the largest, significantly different from big and small fruit of fructus gleditsiae abnormalis. Gleditsia sinensis Lain. and big and small fruit of fructus gleditsiae abnormalis had thick and obvious cell wall, with visible pores in epidermal cells, had palisade cells, seeds and many stone cells, and the difference between Gleditsia sinensis Lain. and small fruit of fructus gleditsiae abnormalis was significant. The contents of crude saponins in Gleditsia sinensis Lain. , small and big fruit of fructus gleditsiae abnormalis were 37.40% ,46.70% ,35.15% respectively,and the contents of echinocyctic acid were 0.37% ,0.11% , 0.27% respectively. The content differences of crude saponins and echinocyctic acid in Gleditsia sinensis Lam., small and big fruit of fructus gleditsiae abnormalis were extremely significant. In summary, Gleditsia sinensis Lain. and fructus gleditsiae abnormalis had significant differences in morphology structure and composition.
作者
李建军
马静潇
尚星晨
张光田
许平
LI Jianjun1 , MA Jingxiao1 , SHANG Xingchen1 , ZHANG Guangtian2, XU Ping3(1. College of Life Science, Henan Normal University/Green Medicine Bioteehnology Henan Engineering Laboratory, Xinxiang 453007, China ; 2. Siehuan Qinba Traditional Chinese Medicine Technology Co. , Ltd. , Bazhong 636600, China; 3. Zoueheng Forestry Bureau, Jining 273500, Chin)
出处
《河南农业科学》
CSCD
北大核心
2018年第7期67-70,共4页
Journal of Henan Agricultural Sciences
基金
中医药公共卫生服务补助专项(财社[2017]66)
河南省教育厅重点研究应用项目(15A180043)
关键词
皂荚
大皂角
猪牙皂
形态结构
粗皂苷
刺囊酸
Gleditsia sinensis
Gleditsia sinensis Lain.
Fructus gleditsiae abnormalis
Morphology struc-ture
Crude saponins
Echinocyctic acid
