摘要
目的拟建立一种高尿酸血症致糖尿病的典型动物模型。方法选取70只12周龄Wistar大鼠,随机分为对照组(A组,30只)和模型组(B组,40只)。A组大鼠给予普通饮食,蒸馏水灌胃。B组大鼠饮食分两个阶段:第一阶段,饲喂高酵母饲料,腺嘌呤溶液(100mg·kg^(-1)·d^(-1))灌胃;在大鼠血尿酸(SUA)水平显著下降时进入第二阶段,此阶段继续喂食高酵母饲料,但灌胃的腺嘌呤溶液剂量改为50 mg·kg^(-1)·d^(-1),同时给予氧嗪酸钾皮下注射。实验期间监测大鼠SUA、血糖、胰岛素等相关生化指标,观察并记录大鼠的进食和排泄情况、体质量变化及精神状态。结果与A组相比,B组大鼠自建模第2周起SUA水平明显升高(t=4.92~16.24,P<0.05);B组大鼠空腹胰岛素及葡萄糖耐量试验(OGTT)2h胰岛素水平随SUA的升高而降低,与A组相比,均自第3周起显现出持续的统计学差异(t=2.679~12.904,P<0.05);与A组相比,B组大鼠空腹血糖及OGTT 2h血糖水平升高,自第10周起表现出统计学差异(t=2.910~13.539,P<0.05)。自第9周起,B组大鼠表现出典型的糖尿病临床症状:多尿、多饮、消瘦、精神萎靡、活动减少。结论造模大鼠的各项指标与临床症状相符,表明高尿酸血症致糖尿病大鼠模型构建成功。
Objective To establish a typical animal model of diabetes induced by hyperuricemia. Methods A total of70 12-week-old Wistar rats were randomly divided into control group（group A,n=30）and model group（group B,n=40）.The rats in group A were given a normal diet and intragastric administration of distilled water.The rats in group B were fed in two stages.In the first stage,high-yeast feed was provided,and adenine solution（100 mg·kg^（-1）·d^（-1））was given by gavage.The second stage of feeding began when the level of rat serum uric acid（SUA）decreased significantly.At this stage,the supply of high-yeast feed continued,but the amount of adenine solution was decreased to 50 mg·kg^（-1）·d^（-1）;meanwhile,subcutaneous injection of potassium oxonate was given to these rats.During the experiment,the rats were monitored for biochemical parameters such as SUA,blood glucose,and insulin,and their food intake,excretion,body weight,and mental status were observed and recorded. Results Compared with group A,group B had a significantly increased level of SUA since the second week of modeling（t=4.92-16.24,P0.05）.In group B,the levels of fasting insulin and oral glucose tolerance test（OGTT）2 hinsulin decreased with the increase in SUA,and both were significantly different from those in group A since the 3 rd week（t=2.679-12.904,P0.05）;the levels of fasting blood glucose and OGTT 2 hblood glucose increased and were significantly different from those in group A since the 10 th week（t=2.910-13.539,P0.05）.Since the 9 th week,the rats in group B showed typical clinical symptoms of diabetes：polyuria,polydipsia,weight loss,low spirit,and decreased activity. Conclusion The indices of the model rats were consistent with the clinical symptoms,which indicates the successful establishment of a rat model of diabetes induced by hyperuricemia.
作者
李传伟
牛佳鹏
阎胜利
孟冬梅
陈西广
邢士超
LI Chuanwei;NIU Jiapeng;YAN Shengli;MENG Dongmei;CHEN Xiguang;XING Shichao(Biochemical and Marine Biological Materials Laboratory,College of Marine Life Sciences,Ocean University of China,Qingdao266003,Chin)
出处
《青岛大学学报（医学版）》
CAS
2018年第4期379-383,共5页
Journal of Qingdao University(Medical Sciences)
基金
国家自然科学基金项目(8167061120
81100554)
山东省优秀中青年科学家基金项目(BS2012YY003)
山东省自然科学基金项目(ZR2014HM015)
山东省医药卫生科技发展计划项目(2016WS0259
2011QZ007)
山东省高校科技发展计划项目(J14LK11)
青岛市科技发展计划项目(12-1-4-20-jch
2012-1-3-2-(1)-nsh
10-3-4-7-5-jch)
青岛经济技术开发区科技发展计划项目(2014-1-72)
青岛市市南区科技发展计划项目(2013-13-008-YY)
