摘要
大气CO_2浓度升高对植物的影响是目前国内外学者普遍关注的问题。为研究CO_2浓度升高对苜蓿(Medicago sativa)不同生长期叶片解剖结构的影响,利用开顶式生长室(open-top chambers,OTC)模拟未来CO_2浓度,以秋眠型、半秋眠型和极非秋眠型的苜蓿为试验材料,采用石蜡切片技术研究苜蓿叶片解剖结构的变化。结果表明,1)叶片解剖结构受CO_2浓度升高、生长期的影响较大,受秋眠类型影响较小;且受三因子交互效应影响显著。2)叶片解剖结构随CO_2浓度升高显著增加(P<0.01),有利于增加叶绿体含量和气体交换速率,进而提高光合作用速率;叶片厚度、表皮厚度、栅栏组织厚度、海绵组织厚度随生长期变化显著增加(P<0.01),但解剖结构在不同秋眠型间差异不大(P>0.05),可能是因为秋眠性受光照时长、气温的影响较大,受CO_2的影响较小。
Effect of elevated atmospheric CO2 concentration on plants has aroused wide concern among scholars.In this study,open-top chambers（OTC）were used to model the future CO2 concentration to study the effect of CO2 enrichment on the leaf anatomical structure of three types fall dormancy at different growth stages by using the paraffin section technique.Results showed that：the anatomical structure of leaves was significantly affected by the increase of CO2 concentration,the growth period,and the interaction effect of three factors.The leaf anatomical structure increased significantly with the increase of CO2 concentration（P〈0.01）,which was beneficial to increase chloroplast content,gas exchange rate,and subsequently the photosynthesis rate.Leaf thickness,cuticle thickness,palisade tissue thickness,and sponge tissue thickness increased significantly with the growth period（P〈0.01）.The anatomical structure of leaves showed no significant differences among three fall dormancy types（P〉0.05）,mainly because fall dormancy is influenced by the duration of light and temperature and is less affected by CO2.The results of this study provide a basis for predicting the effect of elevated CO2 on the anatomical structure of plant leaves for adaptation to CO2 concentration.
作者
杜乐山
刘海燕
翟晓朦
任梦云
李俊生
关潇
Du Le-shan;Liu Hai-yan;Zhai Xiao-meng;Ren Meng-yun;Li Jun-sheng;Guan Xiao(State Environmental Protection Key Laboratory of Regional Eco-process and Function Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,China)
出处
《草业科学》
CAS
CSCD
2018年第7期1704-1712,共9页
Pratacultural Science
基金
环保部生物多样性保护专项(2096001006)
关键词
苜蓿
CO2浓度
秋眠类型
生长期
解剖结构
组织结构紧密度
组织结构疏松度
Medicago sativa
CO2 concentration
fall dormancy
growth period
anatomical structure
cell tense ratio（CTR）
spony ratio（SR）
