摘要
目的制备一种携光敏剂IR780并具有载氧功能的相变型纳米红细胞(Nano-RBCs),探讨其用于体外多模态成像及增强光动力治疗(PDT)的价值。方法通过一步乳化法制备包裹IR780及全氟己烷(PFP)的脂质体(Lip-PFPIR780),负载氧气后即为相变型Nano-RBCs。检测相变型Nano-RBCs的一般理化特性,观察其体外超声/光声/荧光三模态成像效果并进行定量分析,采用单线态氧荧光探针(SOSG)评估其对体外PDT效果的影响。结果制备的Nano-RBCs大小均一,平均粒径(372.5±87.3)nm,IR780包封率92.50%。体外显影实验显示,随相变型Nano-RBCs浓度越高,体外超声、光声、荧光成像效果越好。在IR780浓度一定的情况下,激光照射后相变型Nano-RBCs的SOSG荧光强度明显高于单纯IR780(P<0.05)。结论本研究制备的相变型Nano-RBCs可用于多模态成像,并可增强体外PDT疗效。
Objective To prepare a photosensitizer IR780-based multifunctional nano red blood cells（Nano-RBCs）with oxygen carrying capacity and phase transition,and to investigate its value in multimodal imaging and the enhanced efficacy for photodynamic therapy（PDT）.Methods IR780 and perfluoropentane（PFP）based liposome（Lip-PFP-IR780）was synthesized with a facile one-step emulsion strategy.Nano-RBCs were formed when these liposomes were saturated with oxygen.The characteristics of Nano-RBCs with phase transition were observed.Ultrasound（US）,photoacoustic（PA）and fluorescence（FL）imaging in vitro were performed,and the imaging effect of Nano-RBCs was quantitatively analyzed.In vitro photodynamic properties of Nano-RBCs were evaluated with singlet oxygen sensor green（SOSG）.Results NanoRBCs with phase transition presented homogenized size distribution.The average diameter was（372.50±87.30）nm.Meanwhile,the entrapment efficiency of IR780 was 92.50%.In vitro,the US/PA/FL signal intensities increased along with the increase of Nano-RBCs concentration.Upon the laser irradiation,the fluorescence intensity of SOSG of NanoRBCs with phase transition was higher than that of pure IR780（P〈0.05）.Conclusion Nano-RBCs with phase transition were successfully constructed for multimodal-imaging-guided amplified PDT in vitro.
作者
张亮
杨珂
王冬
王志刚
王颖
陈雪莹
赵钕君
姚元志
ZHANG Liang;YANG Ke;WANG Dong;WANG Zhigang;WANG Ying;CHEN Xueying;ZHAO Nyujun;YAO Yuanzhi(Department of Ultrasound,the First Affiliated Hospital of Chongqing Medical University,Chongqing,400016,China;Institute of Ultrasound Imaging,Chongqing Medical University,Chongqing KeyLaboratory of Ultrasound Molecular Imaging,Chongqing 400010,China;Pediatric ResearchInstitute,Children＇s Hospital of Chongqing Medical University,Chongqing 400014,China;Department of Ultrasound,Chongqing University Cancer Hospital,Chongqing Cancer Institute,Chongqing Cancer Hospital,Chongqing 400030,China)
出处
《中国介入影像与治疗学》
CSCD
北大核心
2018年第8期486-490,共5页
Chinese Journal of Interventional Imaging and Therapy
基金
国家自然科学基金(81571688)
重庆市自然科学基金(cstc2018jcyjAX0526)
关键词
相变
纳米红细胞
光敏剂IR780
全氟己烷
多模态成像
氧
光动力治疗
Phase transition
Nano red blood cells
Photosensitizer IR780
Perfluoropentane
Multimodal imaging
Oxygen
Photodynamic therapy
