摘要
目的:研究胎盘蛋白14(PP14)增强调节性T细胞(Treg细胞)诱导滤泡调节性T细胞(Tfr细胞)增殖的作用及机制。方法:分离健康志愿者外周血中的淋巴细胞;磁珠分选淋巴细胞中的Treg细胞;流式检测CD4^+CD25^+Foxp3Treg细胞比例及其表面CTLA-4和HLA-G的表达;ELISA检测上清中IL-10和TGF-β的浓度;采用Transwell小室进行Treg细胞和淋巴细胞的共培养实验;流式检测淋巴细胞中CD4^+CXCR5^+Foxp3^+Tfr细胞的比例。结果:相对于对照组,PP14组Treg细胞的比例增加了1.26倍(t=4.748,P=0.009 0),而其表面CTLA-4和HLA-G的表达与对照组没有显著的统计学差异(t=0.108,P=0.918 6;t=0.439,P=0.682 8);ELISA的结果显示相对于对照组,PP14组Treg细胞培养上清中IL-10和TGF-β的浓度分别上升了3.66倍和3.86倍(t=6.817,P=0.002 4;t=7.537,P=0.001 7);共培养实验显示PP14能够增强Treg细胞诱导Tfr细胞比例的增加,相对于共培养组,PP14组Tfr细胞比例增加了2.71倍(t=4.815,P=0.008 6),IL-10和TGF-β的阻断均能部分阻断这种增强作用(t=4.868,P=0.008 2;t=3.670,P=0.021 4)。结论:PP14能够通过分泌IL-10和TGF-β,显著增强Treg细胞诱导Tfr细胞比例的增加,这为不明原因复发性流产的治疗提供了一个新的靶点。
Objective：To investigate the effect and mechanism of placental protein 14（PP14） on the proliferation of follicular regulatory T cells（ Tfr cells） induced by regulatory T cells（ Treg cells）. Methods： The lymphocytes in the peripheral blood of the healthy volunteers were separated,and the magnetic beads were selected to separate the Treg cells from the lymphocytes. The ratio of CD4＋CD25＋Foxp3＋Treg cells and the expression of CTLA-4 and HLA-G on their surface were detected by flow cytometry,and the concentration of IL-10 and TGF-β in the supernatant was detected by ELISA. The co-culture experiment of Treg cells and lymphocytes was carried out in the Transwell,and the proportion of CD4＋CXCR5＋Foxp3＋Tfr cells in lymphocytes was detected by flow cytometry. Results：Compared with the control group,the proportion of Treg cells in PP14 group increased by 1. 26 times（ t = 4. 748,P = 0. 009 0）,while the expression of CTLA-4 and HLA-G on their surface was not significantly different（ t = 0. 108,P = 0. 918 6,t =0. 439,P = 0. 682 8）. The results of ELISA showed that the concentration of IL-10 and TGF-β increased by 3. 66 times and 3. 86 times（t= 6. 817,P= 0. 002 4,t= 7. 537,P= 0. 001 7） in PP14 cell Treg supernatant,respectively. Co-culture experiments showed that PP14 could enhance the proliferation of Tfr cells induced by Treg cells,compared with the co-cultured group,the proportion of Tfr cells in PP14 group increased by 2. 71 times（ t = 4. 815,P = 0. 008 6）,blocking IL-10 and TGF-β could partially block this enhancement（ t =4. 868 P = 0. 008 2; t = 3. 670,P = 0. 021 4）. Conclusion：PP14 can significantly enhance the proliferation of Tfr cells induced by Treg cells,which provides a new target for the treatment of unexplained recurrent spontaneous abortion.
作者
林丽慧
陈瑾
唐梅
LIN Li-Hui;CHEN Jin;TANG Mei(Department of Gynaecology and Obstetrics,Hainan West Central Hospital,Danzhou 571700,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2018年第7期1075-1078,共4页
Chinese Journal of Immunology
关键词
胎盘蛋白14
调节性T细胞
滤泡调节性T细胞
不明原因复发性流产
Placental protein 14
Regulatory T cells
Follicular regulatory T cells
Unexplained recurrent spontaneous abortion
