摘要
摘要目的探讨胎盘蛋白14抑制B细胞增殖和分化的作用及机制。方法梯度离心法分离人外周血淋巴细胞；流式检测CD4＋CXCR5＋滤泡辅助性T细胞（Tfh细胞）、CD4＋CXCR5＋叉头螺旋翼状转录因子3（Foxp3）＋滤泡调节性T细胞（Tfr细胞）、CD3-CD19＋B细胞和CD3-CD38＋浆细胞的比例；ELISA法检测上清中IL-21、IL-10和TGF-β的浓度；采用Transwell小室进行细胞的共培养实验；采用t检验进行组间比较。结果对照组Tfh细胞和Tfr细胞的比例分别为（2.52±0.16）%和（1.26±0.24）%，胎盘蛋白14组则分别为（0.84±0.09）%和（4.64±0.68）%，2组之间差异有统计学意义（t=9.150，P=0.000 8和t=4.669，P=0.009 5）；胎盘蛋白14能进一步抑制IL-21分泌（t=5.086，P=0.007 1），而促进IL-10和TGF-β浓度的升高（t=3.599，P=0.022 8和t=6.651，P=0.002 7）；胎盘蛋白14组的B细胞和浆细胞比例分别为（4.87±0.20）%和（5.41±0.54）%，与Tfh细胞组差异有统计学意义（t=4.997，P=0.007 5和t=5.110，P=0.006 9）。结论胎盘蛋白14能够通过抑制Tfh细胞分化和促进Tfr细胞比例增加，进而抑制B细胞的增殖和向浆细胞的分化。
ObjectiveTo investigate the effect and mechanism of placental protein 14 on the proliferation and differentiation of B cells.Methods The lymphocyte of human peripheral blood was separated by gradient centrifugation. Flow cytometry was used to detect the proportion of CD4＋CXCR5＋ follicular helper T cells （Tfh cells）, CD4＋CXCR5＋Foxp3＋ follicular regulatory T cells （Tfr cells）, CD3-CD19＋B cells and CD3-CD38＋ plasma cells. ELISA method was used to detect the concentration of IL-21, IL-10 and TGF-beta in the supernatant, and the co-culture of cells was performed by Transwell chamber; t test was used for comparison between groups.ResultsThe proportion of Tfh cells and Tfr cells in the control group was （2.52±0.16）% and （1.26±0.24）%, respectively, and that of the placental protein 14 groups were （0.84±0.09）% and （4.64±0.68）%, respectively. There was a significant difference between the two groups （t=9.150, P=0.000 8 and t=4.669, P=0.009 5）. Pplacental protein 14 could further inhibit the secretion of IL-21 （t=5.086, P=0.007 1）, and promote the increase of IL-10 and TGF-β concentration （t=3.599, P=0.022 8 and t=6.651, P=0.002 7）. The percentage of B cells and plasma cells in the placental protein 14 group were （4.87±0.20）% and （5.41±0.54）%, which were significantly different from those in the Tfh cell group （t=4.997, P=0.007 5; t=5.110, P=0.006 9）.ConclusionPlacental protein 14 can inhibit the proliferation of B cells and differentiate into plasma cells by inhibiting the differentiation of Tfh cells and increasing the proportion of Tfr cells.
作者
王焕萍
武海英
王瑜
王莉
Wang Huanping;Wu Haiying;Wang Yu;Wang Li(Department of Obstetrics,Henan People＇s Hospital,Zhengzhou 450000,Chin)
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2018年第7期464-466,F0003,共4页
Chinese Journal of Rheumatology
