摘要
从‘霞晖8号’桃花中克隆并鉴定了6个K^+/H^+逆向转运体基因Ppe KEA,在转录水平分析其在桃花发育不同时期的表达模式及对钾肥施用的响应,明确关键基因并验证其功能。结果表明,钾肥施用显著增强盛花期桃花的鲜样质量,提高花朵钾素富集水平。PpeKEA1~PpeKEA6在桃花开放不同时期的表达水平存在差异,均在盛花期达到最高,其转录水平对施用钾肥的响应不同;PpeKEA1在整个桃花开放不同时期的表达量较高,钾肥处理显著增强其在花蕾膨胀期和初开期的表达并抑制其在败落期的表达量;表达载体pDR196-shortKEA1能赋予酵母菌感受态细胞AXT3突变体适应高钾环境而正常生长,说明PpeKEA1的C端区域具有调节桃花组织内K^+转运和平衡的功能。本研究表明PpeKEA1是一个桃花开放过程中调节K^+平衡的K^+/H^+逆向转运体,并可能在桃树钾素营养的动态平衡中起着重要作用。
We isolated six putative PpeKEA（K^＋ efflux antiporter）genes from peach flowers,and analyzed the transcriptional expression and response to potassium（K^＋）fertilizer application during different flowering stages. Key antiporter genes were obtained and functionally verified. K^＋ fertilizer application favorably enhanced the flower fresh weight of‘Xiahui 8＇peach in full bloom stage,and strengthened the flower K^＋ nutritional status. Ppe KEA1–PpeKEA6 were differentially expressed during four stages,and the highest expression level appeared especially in full bloom stage. PpeKEA genes were differentially regulated by K^＋ fertilizer treatment. Notably,PpeKEA1 was the most highly expressed gene throughout the whole flowering process,which was significantly up-regulated in bud period and begin bloom stages but reduced in petal fall stage by K2 O treatment. The recombinant expression vector p DR196-shortKEA1 can mediate K^＋ homeostasis in AXT3 yeast mutant,indicating that expression level of PpeKEA1 was positively correlated with the K^＋ homeostasis in peach flowering process. This study revealed that PpeKEA1 was an important K^＋/H^＋ antiporter that may regulate K^＋ homeostasis during the peach flowering,which contributes to K^＋/H^＋ balance and K^＋ homeostasis in fruit trees.
作者
宋志忠
姜航
张斌斌
马瑞娟
俞明亮
SONG Zhizhong;JIANG Hang;ZHANG Binbin;MA Ruijuan;and YU Mingliang(Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement,Institute of Pomology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2018年第7期1251-1260,共10页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31501743)
江苏省政府留学奖学金项目(JS-2016-190)
国家现代农业产业技术体系建设专项资金项目(CARS-31)
江苏省农业科技自主创新基金项目(CX(15)1020)
