摘要
为建立莽草酸的纯化方法,以莽草酸的含量为指标,对雪松松针水提液的醇沉、大孔吸附树脂单用或与不同柱层析合用的纯化方法进行了探索。最终确定的莽草酸的纯化工艺流程为:提取-醇沉-柱层析-重结晶,其中醇沉法可以将莽草酸的含量由12.69%提升到20.08%,大孔吸附树脂柱层析法单用可以将莽草酸的含量由20.08%提升到35.73%,回收率均超过95%;大孔吸附树脂柱结合硅胶柱层析法可得到纯度为80.05%的莽草酸近白色固体,回收率达60.04%,再经二氯甲烷和甲醇重结晶得到纯度为96.54%的莽草酸白色粉末,表明该纯化方法简单可行,重复性好,易于实施工业化生产。
In order to establish purification method of shikimic acid in water extract from pine leaves of Cedrus deodara.The alcohol precipitation,macroporous adsorption resin column chromatography and macroporous adsorption resin column chromatography binding different column chromatography method were explored with the content of shikimic acid as indexes. The purification process flow of shikimic acid was given as follows： water extraction-alcohol precipitation-column chromatography-recrystallization. Among which the purity of shikimic acid can be increased from 14. 87% to 20. 08% by alcohol precipitation method and increased from 20. 08% to 35. 73% by macroporous adsorption resin column chromatography method which the recovery of shikimic acid was higher than 95%,nearly white solid shikimic acid with the purity of 80. 05% can be obtained by macroporous adsorption resin column chromatography binding silica gel column chromatography method and the recovery of shikimic acid was up to 60. 04%,shikimic acid white power with the purity of96. 54% was obtained by recrystallization with dichloromethane and methanol. The results showed the purification method is simple,rapid,repeated well and suitable for industrial production of shikimic acid from pine leaves of C. deodara.
作者
杜瑞琴
刘东彦
张莉霞
石晓峰
DU Rui-qin;LIU Dong-yan;ZHANG Li-xia;SHI Xiao-feng(Gansu Provincial Cancer Hosptial;Gansu Academy of Medical Science,Lanzhou 730050,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第6期1073-1077,共5页
Natural Product Research and Development
基金
兰州市2016年第二批科技计划(2016-2-65)
兰州市人才创新创业项目(2014-RC-62)
甘肃省科技支撑计划(1204FKCA152)
关键词
雪松松针
莽草酸
纯化
醇沉法
柱层析法
pine leaves of Cedrus deodara
shikimic acid
purification
ethanol precipitation
column chromatography
