摘要
脂肪酸去饱和酶(FAD)是合成不饱和脂肪酸的关键酶,在植物维持各项生命活动、应对生物与非生物胁迫方面具有重要作用。本研究对花生基因组中的FAD基因家族进行鉴定与生物信息学分析,并利用花生转录组数据对花生FAD(Ah FAD)基因的表达模式及可变剪接形式进行分析。结果显示,在花生基因组中共有31个Ah FAD基因,分为6类,Ah SAD基因亚家族有12条序列,AhFAD2有6条序列,Ah FAD3有4条序列,Ah FAD4/5有3条序列,Ah FAD6有2条序列,Ah FAD7/8有4条序列。聚类分析表明,Ah FAD有两大分支:游离Ah SAD分支和膜结合Ah FAD分支;膜结合Ah FAD分支中Ah FAD4/5起源最早,ω-3 Ah FAD由ω-6Ah FAD进化而来;单双子叶聚类于不同分支。表达模式分析表明,Ah SAD、AhFAD2和Ah FAD3在种子中表达水平较高,Ah FAD4/5、Ah FAD6、Ah FAD7/8在叶中表达量最高。可变剪接分析表明,Ah FAD亚家族之间可变剪接形式差异明显,其中有12个Ah FAD基因发生了可变剪接。TTS和TSS是发生最多的可变剪接事件,其次是IR,最少的是AE和ES。
Fatty acid desaturase （FAD）is a key enzyme in the synthesis of unsaturated fatty acids, which plays an important role in maintenance of various life activities of plants and in response to biotic and abiotic stresses.In this study,the peanut FAD （AhFAD）gene family were identified and conducted bioinfor-matics analysis in genome-wide range,and the expression pattern and alternative splicing forms of AhFADs were analyzed using peanut transcriptome data.The results showed that there were 3 1 AhFAD genes in peanut genome,which could be divided into 6 subfamilies.There were 12 members in AhSAD subfamily,6 members in AhFAD2 subfamily,4 members in AhFAD3 subfamily,3 members in AhFAD4/5 subfamily,2 members in AhFAD6 subfamily and 4 members in AhFAD7/8 subfamily.Clustering analysis showed that there were two major branches of AhFAD：free and membrane -binding AhFAD branch.In membrane -binding AhFAD branch,the origin of AhFAD4/5 was the earliest,the ω-3 AhFAD was evolved from ω-6 AhFAD,and the mono-dicotyledonous AhFAD was clustered in different branches.The expression levels of AhSAD,AhFAD2 and AhFAD3 in seeds were higher,while AhFAD4/5,AhFAD6 and AhFAD7/8 had the highest expression in leaves.The analysis of alternative splicing showed that there were significant differences in alternative splicing forms among the subfamilies of AhFAD,of which,12 AhFAD genes had alternative splicing isoforms.TTS and TSS were the most frequent alternative splicing events,followed by IR,and the fewest ones were AE and ES.
作者
阮建
单雷
李新国
郭峰
孟静静
万书波
彭振英
RHan Jian;Shan Lei;Li Xinguo;Guo Feng;Meng Jingjing;Wan Shubo;Peng Zhenying(Biotechnology Research Center,Shandong Academy of Agricultural Scienees/Shandong Provincial Key Laboratory of Crop Genetic Improvement,Ecology and Physiology,Jinan 250100,China;Shandong Academy of Agricultural Sciences,Jinan 250100,China)
出处
《山东农业科学》
2018年第6期1-9,共9页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(31470349
31571605)
国家科技支撑计划项目(2014BAD11B04)
国家国际科技合作专项(2015DFA31190)
国家现代农业产业技术体系建设专项(CARS-13)
