摘要
玉米是喜温作物,但在生长发育过程中对高温天气敏感,易造成产量下降。以耐热玉米品种先甜5号幼苗为实验材料,在42℃下处理0、0.5和3.0 h,提取相应处理的叶片蛋白,进行差异蛋白的筛选,随后对其进行GO分类和KEGG途径分析。结果显示,共筛选到181个差异蛋白,其中上调蛋白95个(定量比值>1.20且P<0.05),下调蛋白86个(定量比值<0.83且P<0.05)。与对照相比,随着热处理时间延长,上调蛋白数明显增加,而下调蛋白数有所下降。GO分类结果显示,2个差异上调蛋白被富集并指定分布在2个细胞组分中,18个差异上调蛋白被富集并指定具有8种分子功能,15个差异上调蛋白被富集并参与17种生物过程。而2个差异下调蛋白被富集并指定分布在8个细胞组分中,29个差异下调蛋白被富集并指定具有11种分子功能,45个差异下调蛋白参与18种生物过程。KEGG分析结果显示,有16个差异上调蛋白被富集并指定参与6个途径,而有14个差异下调蛋白被富集并指定参与8个途径。Domain分析表明,有27个差异上调蛋白被富集并指定含有22个不同结构域,而有34个差异下调蛋白被富集并指定含有21个不同结构域。根据NCBI数据库中信息对上述相关蛋白进行搜索,结果发现假定Dna J分子伴侣蛋白家族、热休克蛋白、硫氧还蛋白H型、脱氢酶和泛素结合酶5类蛋白与耐热关系密切。总之,上述热胁迫响应相关的蛋白质的分析鉴定,可为进一步揭示玉米响应热胁迫的分子机制提供新的思路。
Maize is a warm-tempered crop,but it is sensitive to high temperature weather during its growth and development,which is easy to cause the decline of yield. In this study,seedlings of a heat-resistant maize variety Xiantian No. 5 as experimental materials were treated for 0,0. 5,3. 0 h at 42 ℃,and leaf proteins from all treatments was extracted to carry out screening of differential proteins,and then the GO classification and KEGG pathway were analyzed. The results showed that 181 proteins including 95 up-regulated proteins（ quantitative ratio 〉1. 20 and P〈 0. 05） and 86 down-regulated proteins（ quantitative ratio 0. 83 and P〈 0. 05） were identified. Compared with thecontrol,the number of up-regulated proteins increased with the increase of heat treatment time,while the number of down-regulated proteins decreased. GO classification indicated that in these differentially up-regulation proteins,there were 2 proteins enriched and designated to 2 cell components,18 proteins enriched and designated to 8 molecular functions and 15 proteins enriched and involved in 17 biological processes. Similarly,in differentially down regulated proteins,there were 2 proteins enriched and distributed in 8 cell components,29 proteins were enriched and designated to 11 molecular functions. 45 proteins involved in 18 biological processes. KEGG pathway analysis revealed that 16 differentially up-regulated proteins were enriched and designated to participate in 6 pathways,while 14 differentially down-regulated proteins were enriched and designated to participate in 8 pathways. Domain analysis revealed that 27 differentially up-regulated proteins were enriched and designated to 22 various domains,while 34 differentially down-regulated proteins were enriched and designated to 21 different domains. According to the searching information in NCBI database,we found that 5 proteins of Dna J molecular chaperone family including heat shock protein,thioredoxin H type,dehydrogenase and ubiquitin binding enzyme were likely to be close
作者
石江
赵琳
朱月清
楼旭平
余建忠
阮松林
陈文岳
SHI Jiang;ZHAO Lin;ZHU Yueqing;LOU Xuping;YU Jianzhong;RUAN Songlin;CHEN Wenyue(Hangzhou Academy of Agricuhural Sciences,Hangzhou 310024,China;Lin’an District Forestry Bureau（Ag-ricultural Bureau）of Hangzhou,Hangzhou 311300,China;Agricultural Science and Technology Institute of Xi-aoshan District of Hangzhou City,Hangzhou 311202,China;Agricultural Bureau of Chun’an County of Hangzhou City,Hangzhou 311700,China)
出处
《浙江农业学报》
CSCD
北大核心
2018年第6期893-908,共16页
Acta Agriculturae Zhejiangensis
