摘要
目的探讨转化生长因子β1(TGF-β1)对M2型巨噬细胞诱导淋巴瘤细胞增殖的影响。方法用TGF-β1处理淋巴瘤细胞Jiyoye,MTT测定细胞增殖。用M2型巨噬细胞培养液上清处理淋巴瘤细胞,同时给予TGF-β1刺激,MTT测定细胞增殖,Transwell小室测定细胞迁移和侵袭,Western blot测定基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)、增殖细胞核抗原(PCNA)、细胞核增殖抗原(Ki-67)蛋白水平。结果 TGF-β1作用后的淋巴瘤细胞增殖能力下降,细胞侵袭数目、迁移数目明显降低,细胞中MMP-2、MMP-9、PCNA、Ki-67蛋白水平降低,与未处理的淋巴瘤细胞比较,差异有显著性(P<0.05)。M2型巨噬细胞培养液上清处理后的淋巴瘤细胞增殖能力升高,细胞侵袭数目和迁移数目增多,细胞中MMP-2、MMP-9、PCNA、Ki-67蛋白水平升高,与未处理的淋巴瘤细胞比较,差异有显著性(P<0.05)。淋巴瘤细胞经M2型巨噬细胞培养液上清和TGF-β1处理后细胞增殖能力、细胞侵袭数目、细胞迁移数目及MMP-2、MMP-9、PCNA、Ki-67蛋白水平较单纯TGF-β1处理的细胞相比明显升高,较单纯M2型巨噬细胞培养液上清处理后的细胞相比明显降低,差异有显著性(P<0.05)。结论 TGF-β1可以降低巨噬细胞诱导淋巴瘤细胞增殖、侵袭和迁移,作用机制与MMP-2、MMP-9、PCNA、Ki-67蛋白表达有关。
Objective To investigate the effect of TGF-β1 on the proliferation of lymphoma cells induced by M2macrophages. Methods TGF-β1 was used to treat Jiyoye lymphoma cells, and MTT was used to determine the cellproliferation. Lymphoma cells were treated with M2 macrophage culture liquid supernatant and TGF-β1. MTT assay wasused to assess the lymphoma cell proliferation, Transwell assay was used to assess the cell migration and invasion, andWestern blot was perfomed to determine the levels of MMP-2, MMP-9, PCNA and Ki-67 proteins. Results Thelymphoma cells after TGF-α treatment showed that the proliferation ability, the number of invaded cells and the number ofmigrated cells, and the levels of MMP-2, MMP-9, PCNA and Ki-67 were significantly decreased, compared with the untreated lymphoma cells （P 〈 0. 05）. After treated with the supernatant of M2 type macrophage culture supernatant, theproliferation ability of lymphoma cells, the number of invaded cells and migrated cells, the levels of MMP-2, MMP-9,PCNA and Ki-67 proteins were significantly increased, compared with the untreated lymphoma cells （ P 〈 0. 05 ）.Compared with the lymphoma cells treated with culture medium supernatant of M2 macrophages alone, the lymphoma cellstreated with TGF-β1 and M2 macrophage culture medium supernatant showed that the proliferation ability, number ofinvaded and migrated cells, and the protein levels of MMP-2, MMP-9, PCNA and Ki-67 were significantly increased （P 〈0. 05）. Conclusions TGF-β1 can reduce the proliferation, invasion and migration of lymphoma cells induced bymacrophages. The mechanism of action is related to the expression of MMP-2, MMP-9, PCNA and Ki-67 proteins.
作者
谢瑜
黎承平
武坤
谭琳
XIE Yu;LI Chengping;WU Kun;TAN Lin(Department of Hematology;Department of Clinical Laboratory,the First Affiliated Hospital of KunmingMedical University,Kunming 650032,China)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第6期72-78,共7页
Chinese Journal of Comparative Medicine
