摘要
目的:研究猪苓多糖对IL-4诱导的M2亚型巨噬细胞膜表面分子的阳性表达率的影响和相关炎症因子的作用。方法:实验分为5组:空白对照组,模型组,猪苓多糖低、中、高剂量组(50、100、200μg/ml)。用20 ng/ml的IL-4诱导为M2亚型巨噬细胞,采用流式细胞术检测CD206、CD16/32阳性表达率,实时荧光定量PCR(qRT-PCR)检测相关因子mRNA表达量,造模成功后予猪苓多糖干预M2亚型巨噬细胞,检测猪苓多糖对巨噬细胞膜分子的影响和相关因子的mRNA的影响。结果:IL-4可以稳定建立M2亚型巨噬细胞模型,猪苓多糖可以明显提高CD16/32、CD40的阳性表达率,并显著增加相关炎症因子的表达率。结论:猪苓多糖可以逆转M2亚型巨噬细胞为M1巨噬细胞,提高巨噬细胞的免疫功能。
Objective： To study the positive expression rate of M2 subtype of macrophage cell surface molecules and the inflammatory factors of PPS in IL-4-induced M2 macrophage. Methods： The experiment was divided into 5 groups： blank control group,Model group,PPS groups（ 50 μg / ml,100 μg / ml and 200 μg / ml）. The expression of CD206 and CD23 was used as bio-maker to confirm IL-4induced macrophages by treating RAW264. 7 with 20 ng / ml of IL-4. IL-4 induced RAW264. 7 cells were treated with PPS of 50 μg / ml,100 μg / ml and 200 μg / ml for 24 h. Then the expression of CD206,CD16 /32 and CD40 were analyzed by flow cytometry,and the mRNA expression of IL-1β,TNF-α,IL-10 and i NOS were detect by qRT-PCR. Results： After treated with IL-4,the positive rate of CD206 of RAW264. 7 were high. After treated with PPS,the rate of CD16 /32 and CD40 in IL-4 induced RAW264. 7 cells were high,the expression of CD206 decreased,and the mRNA level of IL-1β and TNF-α increased. Conclusion： RAW264. 7 cells can be polarlized to M2 subtype macrophage by using 20 ng / ml IL-4. PPS enhances the mRNA of IL-1β,TNF-α and the expression of CD40,CD16 /32 in IL-4-induced RAW264. 7 cells. These results indicate that PPS can induce the M2 subtype to become M1 macrophages,can improve immune function of macrophages.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第8期1049-1052,共4页
Chinese Journal of Immunology
基金
国家科技部重大新药创制项目(No.2012ZX09303009-003)
广东省科技计划项目(No.2010B030700059)
广东省自然科学基金(No.2014A03031345)
