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Determination of Content of Amino Acid in Abalone by Precolumn Derivatization and its Nutritional Value Evaluation 被引量:5

Determination of Content of Amino Acid in Abalone by Precolumn Derivatization and its Nutritional Value Evaluation
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摘要 [Objectives] This study was conducted to optimize the determination conditions of amino acids from abalone. [Methods] The sample was treated by acid hydrolysis method and subjected to 2,4-2 nitro fluorobenzene column derivatization. The amino acid content in abalone was determined by HLPC,and the nutritional value of the amino acids was evaluated with egg protein model put forward by Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine. [Results] Abalone contains full amino acids. According to the FAO/WHO ideal,it is a high-quality protein source and suitable for supplement of protein source for human body. [Conclusions]The experimental method has simple operation and could achieve a good effect with wide linear range and correlation coefficient over 0. 999 8,and the obtained results are satisfactory. [Objectives] This study was conducted to optimize the determination conditions of amino acids from abalone. [Methods] The sample was treated by acid hydrolysis method and subjected to 2,4-2 nitro fluorobenzene column derivatization. The amino acid content in abalone was determined by HLPC,and the nutritional value of the amino acids was evaluated with egg protein model put forward by Institute of Nutrition and Food Hygiene,Chinese Academy of Preventive Medicine. [Results] Abalone contains full amino acids. According to the FAO/WHO ideal,it is a high-quality protein source and suitable for supplement of protein source for human body. [Conclusions]The experimental method has simple operation and could achieve a good effect with wide linear range and correlation coefficient over 0. 999 8,and the obtained results are satisfactory.
出处 《Agricultural Biotechnology》 CAS 2018年第3期178-183,210,共7页 农业生物技术(英文版)
基金 Supported by National Natural Science Foundation of Hainan Province(217157) Fund for Construction of Hainan Provincial Key Discipline(Thalassochemistry) in 2017
关键词 ABALONE Precolunm derivatization HPLC Amino acid EVALUATION Abalone Precolunm derivatization HPLC Amino acid Evaluation
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