摘要
目的:研究阿魏酸对高脂饮食结合小剂量链脲佐菌素(STZ)诱导的2型糖尿病小鼠心肌病变的影响,探讨其可能的作用机制。方法:雄性ICR小鼠20只,高糖高脂饮食连续6周,STZ(30 mg/kg)腹腔注射连续5d后,隔9d测空腹血糖(FBG),超过11.1 mmol/L视为糖尿病造模成功。将此20只小鼠随机分为模型组、阿魏酸组(200mg/kg,i.g.),每组10只;另取10只正常小鼠为对照组;连续给药8周。末次给药后,测定FBG、称体重、全心重和左心室重,计算心脏质量指数(HMI)和左室质量指数(LVMI);测定超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量;Masson染色观察左心室纤维增生情况;免疫组化法测定心肌组织转化生长因子-β1(TGF-β1)、Ⅲ型胶原蛋白表达。结果:与模型组小鼠比较,阿魏酸组小鼠HMI、LVMI减小((P<0.01,P<0.05),心肌组织SOD活力升高、MDA含量下降(P<0.05);心肌胶原纤维沉积减少,间质纤维化改善;免疫组化显示心肌组织TGF-β1和Ⅲ型胶原蛋白表达减少(P<0.01,P<0.05)。结论:阿魏酸对糖尿病小鼠心肌病变具有保护作用,可能与抗氧化及抑制TGF-β1蛋白表达,减少Ⅲ型胶原蛋白沉积有关。
Objective： To study the effect of ferulic acid on diabetic cardiomyopathy of type 2 diabetic mice induced by high fat diets combined with low dose streptozotocin（ STZ）,and to explore its possible mechanism. Methods： Twenty male ICR mice were fed with high fat diets for 6 weeks,and then were injected intraperitoneally with STZ（ 30 mg/kg） for 5 continuous days. Nine days later,the fasting blood glucose（ FBG） was detected,mouse with FBG over 11. 1 mmol/L was considered as diabetes mellitus. Twenty diabetic mice were randomly divided into two groups： model group and ferulic acid group（ 200 mg/kg,i. g.）,10 in each group. Taking another ten age and sex matched mice as a control group. After continuous administration for 8 weeks,FBG,body weight,heart weight,left ventricular weight,the activity of superoxide dismutase（ SOD） and the content of malondialdehyde（ MDA） were detected,the heart mass index（ HMI） and left ventricular mass index（ LVMI） were calculated. Masson staining was employed to observe pathological changes; immunohistochemistry was used to determine the expression of transforming growth factor-β1（ TGF-β1）,collagen III in the myocardial tissue. Results： Compared with the model group,HMI,LVMI,the content of MDA were significantly decreased（ P 〈0. 01,P 〈0. 05） and the activity of SOD was notably increased（ P〈 0. 05） in the ferulic acid group. Collagen deposition and interstitial fibrosis in myocardial tissue were improved. Immunohistochemistry showed that expressions of TGF-β1 and type III collagen remarkably decreased（ P 〈0. 01,P 〈0. 05） in myocardial tissue. Conclusion： Ferulic acid has a protective effect on diabetic cardiomyopathy in diabetic mice,and its mechanism may be linked to anti-oxidation,inhibiting the expression of TGF-β1 protein and reducing the deposition of production oftypeⅢcollagen.
作者
徐慧琳
徐郭王君
姚君
郭玮
项楠
王旭焘
齐敏友
XU Hui-lin;XU Guo-jun;YAO Jun;GUO Wei;XIANG Nan;WANG Xu-tao;QI Min-you(Institution of Pharmacology,College of Pharmaceutical Sciences,Zhejiang University of Technology,Hangzhou 310014)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2018年第3期238-241,共4页
Chinese Journal of Applied Physiology
基金
浙江省自然科学基金(LY16H280013)
浙江工业大学大学生创新创业训练计划(2017094)
关键词
阿魏酸
糖尿病心肌病
氧化应激
纤维化
小鼠
fenllic acid
diabetic cardiomyopathy
oxidative stress
fibrosis
mice
