摘要
目的建立HPLC-DAD法同时测定益肾健骨丸(当归、枸杞子、苏木等)中5种成分的含量。方法该药物甲醇提取液的分析采用Waters Symmetry C18色谱柱(250 mm×4.6 mm,5μm);流动相甲醇-0.2%磷酸,梯度洗脱;体积流量1 mL/min;检测波长230、330 nm;柱温25℃。结果龙胆苦苷、马钱苷、芍药苷、阿魏酸、毛蕊花糖苷分别在63.60~636.00μg/mL(r=0.9999)、5.90~59.00μg/mL(r=0.9996)、40.125~401.25μg/mL(r=0.9997)、2.0625~20.625μg/mL(r=0.9999)、5.775~57.75μg/mL(r=0.9998)范围内线性关系良好,平均加样回收率分别为100.5%、99.6%、100.4%、100.1%、99.57%,RSD分别为1.05%、1.36%、1.19%、1.55%、1.24%。结论该方法简便可靠,重复性好,可用于益肾健骨丸的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of five constituents in Yishen Jiangu Pills(Angelicae sinensis Radix,Lycii Fructus,Sappan Lignum,etc.).METHODS The analysis of methanol extract of this drug was performed on a 25℃thermostatic Waters Symmetry C18 column(250 mm×4.6 mm,5μm),with the mobile phase comprising of methanol-0.2%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 230,330 nm.RESULTS Gentiopicroside,logain,paeoniflorin,ferulic acid and verbascoside showed good linear relationships within the ranges of 63.60-636.00μg/mL(r=0.9999),5.90-59.00μg/mL(r=0.9996),40.125-401.25μg/mL(r=0.9997),2.0625-20.625μg/mL(r=0.9999)and 5.775-57.75μg/mL(r=0.9998),whose average recoveries were 100.5%,99.6%,100.4%,100.1%and 99.57%with the RSDs of 1.05%,1.36%,1.19%,1.55%and 1.24%,respectively.CONCLUSION This simple,reliable and reproducible method can be used for the quality control of Yishen Jiangu Pills.
作者
王雪梅
刘校栓
王宝才
李喜香
李盛华
戴刚
WANG Xue-mei;LIU Xiao-shuan;WANG Bao-cai;LI Xi-xiang;LI Sheng-hua;DAI Gang(Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730000,China)
出处
《中成药》
CAS
CSCD
北大核心
2021年第1期34-38,共5页
Chinese Traditional Patent Medicine
基金
中医药行业科研专项(201507001-06)。
