摘要
目的:采用响应面法优化黄芪茎叶总黄酮的提取条件,并对其抗炎功效进行评价。方法:在单因素试验的基础上,通过Box-Behnken试验设计,获得总黄酮的最佳提取工艺,并采用小鼠巨噬细胞构建体外炎症模型研究其抗炎活性。结果:黄芪茎叶总黄酮最佳提取条件为乙醇浓度80%、液料比15∶1、提取温度70℃、超声功率200 W,在此条件下总黄酮得率为8.58%;黄芪茎叶总黄酮能够显著降低小鼠巨噬细胞本底NO水平,并抑制由LPS所诱导的NO释放量的增加。结论:利用响应面法分析结果可靠,所得提取工艺合理可行,且黄芪茎叶总黄酮具有明显的抗炎活性。
Objective： To explore the optimal extraction conditions of total flavonoids from stems and leaves of Astragalus membranaceus by response surface methodology and evaluate the anti-inflammatory activity. Methods： Based on the single-factor tests,extraction conditions of total flavonoids were optimized by Box-Behnken design,and the anti-inflammatory activity was assessed by in vitro inflammatory model of mouse macrophages. Results： The optimal extraction conditions of total flavonoids were ethanol concentration of 80%,liquid-material ratio of 15：1,extraction temperature of 62 ℃ and ultrasonic power of 200 W,the maximum extraction rate of total flavonoids was 8. 58% under the conditions. Total flavonoids from stems and leaves of A. membranaceus reduced background NO level of mouse macrophages significantly,and inhibited the production of NO induced by LPS. Conclusion： The results of response surface methodology are reliable and extraction conditions are applicable. The total flavonoids from stems and leaves of A. membranaceus have strong anti-inflammatory activity.
作者
黄文静
孙晓春
周瑞
张严磊
谢培
李铂
王二欢
唐志书
HUANG Wen-jing;SUN Xiao-chun;ZHOU Rui;ZHANG Yan-lei;XIE Pei;LI Bo;WANG Er-huan;TANG Zhi-shu(Shaanxi University of Chinese Medicine/Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Xianyang 712083,China;Shaanxi Buchang Pharmaceutical Limited Company,Xi＇an 710077,China)
出处
《中国现代中药》
CAS
2018年第7期871-877,共7页
Modern Chinese Medicine
基金
国家中药标准化项目(ZYBZH-C-QIN-45)
国家自然科学基金项目(31600320)
现代农业产业技术体系建设专项资金资助(CARS-21)
陕西省中医药管理局2017-2018年度中医药科研项目(ZYMS002)
关键词
黄芪茎叶
总黄酮
响应面法
提取工艺
抗炎活性
Stems and leaves of Astragalus membranaceus
total flavonoids
response surface methodology
extractionprocess
anti-inflammatory activity
