摘要
目的研究树突状细胞 （DC）-细胞因子引导的杀伤细胞（CIK） 联合γ-干扰素（IFN-γ）对慢性乙型病毒性肝炎的治疗效果。方法取患者自体外周血，培养自体DC-CIK，乙型肝炎病毒表面抗原（HBsAg）活化后回输至慢性乙型病毒性肝炎患者体内。同时予以IFN-γ皮下注射治疗48周。检测治疗前和治疗后24周患者外周血乙肝病毒脱氧核糖核酸（HBV-DNA）和丙氨酸氨基转移酶（ALT）水平。以Fibroscan肝纤维化无创检测仪检测治疗前后肝硬度值。结果DC-CIK联合IFN-γ治疗后慢性乙型病毒性肝炎患者外周血HBV-DNA水平明显下降（7.04±1.02比5.62±1.99，P＝0.001），病毒学应答率为56.7%。DC-CIK联合IFN-γ治疗后肝脏硬度值明显下降（9.65±3.75比6.63±2.48，P＝0.000）。结论DC-CIK联合IFN-γ治疗可抑制乙肝病毒复制并抑制慢性乙型病毒性肝炎患者肝纤维化过程。
ObjectiveTo investigate the therapeutic effect of dendritic cells （DC）-cytokine guided killer cells （CIK） combined with interferon-γ （IFN-γ） on chronic viral hepatitis type B. MethodsAutologous peripheral blood was extracted from the patients to culture autologous DC-CIK. Hepatitis B virus surface antigen （HBsAg） was activated and then returned into chronic viral hepatitis type B patients. At the same time, IFN-γ was given for 48 weeks by subcutaneous injection. The levels of hepatitis B virus-DNA （HBV-DNA） and alanine aminotransferase （ALT） in the peripheral blood of the patients before and 24 weeks after treatment were detected. Liver stiffness was measured before and after treatment with Fibroscan liver fibrosis noninvasive detector. ResultsAfter DC-CIK combined with IFN- γ treatment, the level of HBV-DNA in peripheral blood of patients with chronic viral hepatitis type B was significantly decreased （7.04±1.02 vs. 5.62±1.99, P=0.001）, and the response rate of virology was 56.7%. After DC-CIK combined with IFN-γ treatment, the liver hardness decreased significantly （9.65±3.75 vs. 6.63±2.48, P=0.000）. ConclusionsDC-CIK combined with IFN-γ can inhibit hepatitis B virus replication and inhibit liver fibrosis in patients with chronic viral hepatitis type B.
作者
韩际奥
马英杰
郭云霞
秦宝山
张意兰
白璐
Han Ji＇ ao;Ma Yingfie;Guo Yunxia;Qin Baoshan;Zhang Yilan;Bai Lu(Department of Gastroenterology,Zhengzhou People＇s Hospital,Institute of Liver and Gastrointestinal Dis- ease of Zhengzhou,Zhengzhou 450003,Chin)
出处
《中国实用医刊》
2018年第13期28-31,共4页
Chinese Journal of Practical Medicine
基金
河南省科技攻关基金资助项目（152102310002）
