摘要
建立了兽用板蓝根颗粒中腺苷含量测定的高效液相色谱法。以Symmetry C18柱为固定相,甲醇-0.05 mol/L磷酸二氢钾溶液(10∶90)为流动相,检测波长260 nm,流速为1.2 m L/min,柱温30℃。采用外标法定量计算含量。腺苷的线性范围为4~20μg/m L,回归方程y=71169x-6732,R2=0.9999;平均回收率96.62%,RSD为1.08%。方法操作简单、精密度高、稳定性好,测定结果准确可靠,可用于板蓝根颗粒中腺苷的质量控制。
A method was developed to determine the content of Adenosine in Banlangen Granule by HPLC,with Symmetry C（18） column as stationary phase,mobile phase was methyl alcohol-0. 05 mol/L potassium dihydrogen phosphate（10 ∶ 90）,detection wavelength was 260 nm,flow rate was 1.2 m L/min,and column temperature was30 ℃. It was quantified by external standard method and adenosine in sample quantity is 4 - 20 μg/m L range,show a good linear relationship,y = 71169 x-6732（ R^2= 0.9999）. The recovery was 96.62%,RSD was 1.08%.This method is simple and accurate with good stability,it is suitable for determining the content of Adenosine in Banlangen Granule.
作者
王亚芳
钟昆芮
李应超
周艳飞
张小飞
高婷
秦俊杰
王海军
战皓
WANG Ya-fang;ZHONG Kun-rui;LI Ying-chao;ZHOU Yan-fei;ZHANG Xiao-fei;GAO Ting;QIN Jun-jie;WANG Hai-jun;ZHAN Hao(Beijing Institute of Veterinary Drug Control,Beifing 100107,China;Beijing Centre Biology Co.Ltd.,Beijing 102206,China;Beijing Veterinary Drugs Engineering Research Center,Beijing 102206,China)
出处
《中国兽药杂志》
2018年第7期59-63,共5页
Chinese Journal of Veterinary Drug
