摘要
目的:建立一测多评法测定白背叶药材中3个黄酮碳苷成分葫芦巴苷Ⅱ、夏佛托苷和异夏佛托苷的含量,并验证其准确性及可行性。方法:采用高效液相色谱法,使用C_(18)色谱柱(4.6 mm×250 mm,5μm),柱温25℃,流动相为甲醇-0.1%乙酸水溶液,梯度洗脱,流速1.0 m L·min^(-1),检测波长336 nm。以葫芦巴苷Ⅱ为内参物,建立其与夏佛托苷和异夏佛托苷的相对校准因子,计算白背叶药材中夏佛托苷、异夏佛托苷的含量,实现一测多评。同时采用外标法测定白背叶药材中3个黄酮碳苷成分的量,比较计算值与测定值之间的差异,以验证一测多评法在测定中的准确性及可行性。结果:在一定线性范围内,葫芦巴苷Ⅱ与夏佛托苷、异夏佛托苷的相对校正因子分别为1.315、1.022。一测多评法测得6批样品中葫芦巴苷Ⅱ的含量范围为0.75~7.51 mg·g^(-1),夏佛托苷的含量范围为1.24~7.56 mg·g^(-1),异夏佛托苷的含量范围为1.03~5.99 mg·g^(-1),与外标法测定结果无显著性差异。结论:所建立的同时测定葫芦巴苷II、夏佛托苷和异夏佛托苷含量的一测多评法准确、可行,可用于白背叶药材的定量分析。
Objective：To develop a method for the simultaneous determination of three flavone C-glycosides（viceninⅡ,schaftoside and isoschaftoside）in the leaves of Mallotus apelta（Lour.）Muell.Arg.by quantitative analysis of multi-components by single-marker（QAMS）,and to verify accuracy and feasibility of the method.Methods：HPLC was performed on a C_（18） analytical column（4.6 mm×250 mm,5μm）at 25℃with methanol and 0.1%acetic acid as the mobile phase with gradient elution.ViceninⅡwas used as the internal reference substance.The relative correlation factors（RCFs）of schaftoside and isoschaftoside to viceninⅡwere determined.The contents of these three flavone C-glycosides were determined by the external standard method（ESM）and QAMS method.The QAMS method was validated through comparison of the results obtained by the two different methods.Results：RCFs of schaftoside and isoschaftoside to viceninⅡwere 1.315 and 1.022 respectively.The content ranges of viceninⅡ,schaftoside and isoschaftoside by the QAMS method were 0.75-7.51 mg·g^（-1）,1.24-7.56 mg·g^（-1） and 1.03-5.99 mg·g^（-1）,respectively.The two methods did not show significant difference in assay results and the RCFs were reliable.Conclusion：The QAMS method for simultaneous determination of viceninⅡ,schaftoside and isoschaftoside is feasible,accurate,and can be used to determine three flavone C-glycosides in the leaves of M.apelta.
作者
成林
谢巍
朱斌
蒋受军
CHENG Lin;XIE Wei;ZHU Bin;JIANG Shou-jun(Guangxi Nanning Municipal Red Cross Hospital,Nanning 530012,China;Women and Children＇s Health Care Hospital of Liuzhou,Liuzhou 545001,China;Guangxi Institute for Food and Drug Control,Nanning 53002 l,China;Center for ADR Monitoring of Guangxi,Nanning 530029,China)
出处
《药物分析杂志》
CAS
CSCD
北大核心
2018年第7期1152-1157,共6页
Chinese Journal of Pharmaceutical Analysis
基金
广西自然基金项目"广西特色民族药白背叶物质基础
质量控制及活性成分药代动力学研究"(编号2010GXNSFA013233)
关键词
白背叶
黄酮碳苷类成分
芹菜素结构母核
葫芦巴苷Ⅱ
夏佛托苷
异夏佛托苷
一测多评法
Mallotus apelta
flavone C-glycosides
common structure of apigenin
vicenin II
schaftoside
isoschaftoside
quantitative analysis of multi-components by single-marker （ QAMS ）
